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001-es BibID:	BIBFORM053643
Első szerző:	Kiss Flóra (bőrgyógyász)
Cím:	Leukemic lymphoblasts, a novel expression site of coagulation factor XIII subunit A / Flóra Kiss, Zsuzsanna Hevessy, Anikó Veszprémi, Éva Katona, Csongor Kiss, György Vereb, László Muszbek, János Kappelmayer
Dátum:	2006
Megjegyzések:	Blood coagulation factor XIII (FXIII) is a protransglutaminasecirculating as a tetramer formed by two types of subunits (A2B2).The intracellular dimeric form of FXIII (A2) is present in platelets,megakaryocytes, monocytes and macrophages and hasbeen detected in mono- and megakaryocytic leukemias.The aimof our study was to investigate FXIII-A expression in newly diagnosedB cell acute lymphoblastic leukemia (ALL) samples. Weexamined 47 de novo ALL cases of B cell origin by triple colorlabeling with flow cytometry. FXIII-A was detected by a FITCconjugated monoclonal antibody combined with CD34 andCD45 staining. In selected cases FXIII-A was investigated onslides prepared from blasts and visualized with a fluorescentmicroscope. In addition, blasts were studied by Western blotanalysis and FXIII-A was measured by a highly sensitive ELISAmethod. By flow cytometry 19 samples of the 47 cases werefound to be FXIII-A positive. Antigen concentration was 3.11 ?1.19 fg/blast, while normal lymphoid precursors and maturelymphocytes from B-CLL did not contain FXIII-A.In the lysate oflymphoblasts that were positive by flow cytometry, a single band(82 kDa) corresponding to FXIII-A was detected on Westernblots. Confocal laser scanning microscopic examination revealedthe presence of FXIII-A in the cytoplasm of these lymphoblasts.This novel expression site of FXIII-A in leukemic lymphoblastscan be utilized as a diagnostic tool and may also gain functionalsignificance in B-lineage ALL.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Coagulation factor XIII acute lymphoblastic leukemia flow cytometry
Megjelenés:	Thrombosis Haemostasis. - 96 : 2 (2006), p. 176-182. -
További szerzők:	Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Veszprémi Anikó Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Vereb György (1965-) (biofizikus, orvos) Muszbek László (1942-) (haematológus, kutató orvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM005605
Első szerző:	Kiss Flóra (bőrgyógyász)
Cím:	A coagulation factor becomes useful in the study of acute leukemias : studies with blood coagulation factor XIII / Kiss F., Simon Á., Csáthy L., Hevessy Z., Katona É., Kiss C., Kappelmayer J.
Dátum:	2008
Megjegyzések:	The intracellular form of the coagulation factor XIII has previously been identified by immunomorphological techniques using polyclonal antibodies. In these studies, only the A subunit (FXIII-A) was detectable in megakaryocytes/platelets and in monocytes/ macrophages. We developed several novel monoclonal antibody clones directed to both subunits (FXIII-A and FXIII-B) and investigated their appearance in normal and leukemic cells. By using 3- and 4-color flow cytometry FXIII expression was investigated in normal peripheral blood and bone marrow samples and in acute myeloblastic (AML) and lymphoblastic (ALL) leukemia cases. Samples were studied by Western blotting and confocal laser scanning microscopy. With a previously published ELISA assay applying two monoclonal antibodies directed to different epitopes in FXIII-A, we were able to measure the intracytoplasmic content of FXIII-A in normal cells and leukemic blasts. FXIII-A was detectable in normal peripheral blood monocytes and in large quantities in platelets, but both cell types were negative for FXIII-B. There was no surface staining for FXIII-A, it only appeared intracellularly. In samples derived from patients with AML M4 and M5, FXIII-A sensitively identified blast cells. Although normal lymphocytes do not express FXIII-A, 40% of ALL cases showed significant FXIII-A expression as determined by flow cytometry. FXIII-A positivity of lymphoblasts was verified by Western blotting, ELISA, and confocal laser scanning microscopy cytometry. These data provide evidence that FXIII-A is a sufficiently sensitive marker in differentiating myeloblasts and monoblasts and is suitable for identifying leukemia-associated phenotypes in ALL.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban factor XIII flow cytometry acute leukemia phenotype
Megjelenés:	Cytometry. Part A. - 73A : 3 (2008), p. 194-201. -
További szerzők:	Simon Ágnes (1969-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:	DOI elektronikus változat
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