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001-es BibID:	BIBFORM028544
Első szerző:	Kerékgyártó Csilla
Cím:	Strain differences in the cytotoxic activity and TNF production of murine macrophages stimulated by lentinan / Csilla Kerékgyártó, László Virág, László Tankó, Goro Chihara, Jozsef Fachet
Dátum:	1996
Megjegyzések:	The effect of lentinan, a glucan type immunomodulatory polysaccharide was studied on the antitumor cytotoxicity and on the TNF secretion of peritoneal macrophages in inbred H-2 congeneic mouse strains under in vivo and in vitro conditions. The cytotoxic activity and TNF secretion of murine macrophages was found to be elevated by lentinan in vitro and in vivo conditions. The effectiveness of lentinan to induce cytotoxicity and TNF secretion was highly influenced by the genotype of the host. The increased cytotoxicity of macrophages was modified by the H-2 and the background genes. The black background and the H-2a and H-2d haplotypes were associated with high responsiveness, while the albino and agouti background and the H-2b and the H-2k haplotypes with low responsiveness to lentinan treatment. The degree of TNF secretion of macrophages stimulated by lentinan was influenced by the H-2 genes only. Similarly, to the macrophage cytotoxicity the TNF secretion in the H-2a and H-2d haplotypes were found to be high, on the other hand, in the H-2b and H-2k were low.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban TNF macrophage lentinan egyetemen (Magyarországon) készült közlemény
Megjelenés:	International Journal of Immunopharmacology 18 : 6-7 (1996), p. 347-353. -
További szerzők:	Virág László (1965-) (biokémikus, sejtbiológus, farmakológus) Tankó László Chihara, Goro Fachet József (1935-2016) (immunológus, kórélettanász)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM028545
Első szerző:	Virág László (biokémikus, sejtbiológus, farmakológus)
Cím:	A simple, rapid and sensitive fluorimetric assay for the measurement of cell-mediated cytotoxicity / László Virág, Csilla Kerékgyártó, József Fachet
Dátum:	1995
ISSN:	0022-1759
Megjegyzések:	A fluorimetric method using 4-methylumbelliferyl heptanoate (MUH) has been developed for detecting cell-mediated cytotoxicity and cell proliferation. The assay is based on the hydrolysis of the fluorochrome (MUH) by intracellular esterases of viable cells resulting in the production of highly fluorescent 4-methylumbelliferone that can be measured in a microplate fluorimeter. Because of a similarity to the principle of the widely used colorimetric MTT assay, a comparison was made between the two assays when measuring cell proliferation and LAK cell cytotoxicity to different target cell types. The results have shown that the MUH assay represents a method for evaluating both cell-mediated cytotoxicity and cell proliferation which is completely comparable to the MTT method. The rapidity of the new cytotoxicity assay, 5 h in contrast to 9 h for the MTT assay, its applicability to both adherently and nonadherently growing target cells and its high accuracy due to the avoidance of centrifugation steps make this method a serious contender for replacing conventional radioactive techniques.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Immunological Methods. - 185 : 2 (1995), p. 199-208. -
További szerzők:	Kerékgyártó Csilla Fachet József (1935-2016) (immunológus, kórélettanász)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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