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001-es BibID:BIBFORM118924
035-os BibID:(cikkazonosító)1581 (Scopus)85184721995 (WoS)001161185700001
Első szerző:Bácsi Attila (immunológus)
Cím:Radiation-Detoxified Form of Endotoxin Effectively Activates Th1 Responses and Attenuates Ragweed-Induced Th2-Type Airway Inflammation in Mice / Attila Bácsi, Beatrix Ágics, Kitti Pázmándi, Béla Kocsis, Viktor Sándor, Lóránd Bertók, Géza Bruckner, Sándor Sipka
Dátum:2024
ISSN:1422-0067
Megjegyzések:Urbanization with reduced microbial exposure is associated with an increased burden of asthma and atopic symptoms. Conversely, environmental exposure to endotoxins in childhood can protect against the development of allergies. Our study aimed to investigate whether the renaturation of the indoor environment with aerosolized radiation-detoxified lipopolysaccharide (RD-LPS) has a preventative effect against the development of ragweed-induced Th2-type airway inflammation. To explore this, cages of six-week-old BALB/c mice were treated daily with aerosolized native LPS (N-LPS) or RD-LPS. After a 10-week treatment period, mice were sensitized and challenged with ragweed pollen extract, and inflammatory cell infiltration into the airways was observed. As dendritic cells (DCs) play a crucial role in the polarization of T-cell responses, in our in vitro experiments, the effects of N-LPS and RD-LPS were compared on human monocyte-derived DCs (moDCs). Mice in RD-LPS-rich milieu developed significantly less allergic airway inflammation than mice in N-LPS-rich or common environments. The results of our in vitro experiments demonstrate that RD-LPS-exposed moDCs have a higher Th1-polarizing capacity than moDCs exposed to N-LPS. Consequently, we suppose that the aerosolized, non-toxic RD-LPS applied in early life for the renaturation of urban indoors may be suitable for the prevention of Th2-mediated allergies in childhood.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
endotoxin
lipopolysaccharide
radiation-detoxified
Th2-type airway inflammation
allergy
dendritic cells
ragweed
mice
Megjelenés:International Journal Of Molecular Sciences. - 25 : 3 (2024), p. 1-22. -
További szerzők:Ágics Beatrix (1995-) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus) Kocsis Béla Sándor Viktor Bertók Lóránd (1934-) (sugárbiológus, immunológus) Bruckner Géza Sipka Sándor (1945-) (laboratóriumi szakorvos)
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2.

001-es BibID:BIBFORM076593
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:Comparison of endotoxin levels in cow's milk samples derived from farms and shops / Sipka Sándor, Béres Andrea, Bertók Lóránd, Varga Tamara, Bruckner Geza
Dátum:2015
ISSN:1753-4259 1753-4267
Megjegyzések:The observations on the protective effect of bacterial endotoxin in farm-derived cow's milk on childhood asthma and allergy are contradictory. The aim of this study was to determine the endotoxin levels in ♭farm-derived whole raw' and ♭processed shop' sources of cow's milk, and to test how the temperature and storing conditions might alter their endotoxin concentrations. Milk was collected from farms and shops. The level of endotoxin was measured by micro (gel-clot) Limulus amebocyte lysate test expressed as EU/ml. The concentration ranges of endotoxin were much higher and more widely scattered in the samples of whole raw farm milk than in the processed shop milk. Cold storage or heating increased the endotoxin concentrations in all samples of farm milk, but not in the processed shop milk. These results show that elevated levels of endotoxin in raw farm milk samples can occur from the cowshed or be formed during storage. In processed shop milk, storage does not cause any changes in the amount of endotoxin. Therefore, it is consistent that the handling and storage of raw milk alters the endotoxin concentrations, which may explain previous contradictory findings regarding the beneficial modulating effects on innate immunity toward allergy prevention in early childhood.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Allergy prevention
cow's milk
endotoxin
temperature dependence
Megjelenés:Innate Immunity. - 21 : 5 (2015), p. 531-536. -
További szerzők:Béres Andrea Bertók Lóránd (1934-) (sugárbiológus, immunológus) Varga Tamara Bruckner Géza
Pályázati támogatás:71883
OTKA
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3.

001-es BibID:BIBFORM076595
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:Effects of L-amino Acids on Human Peripheral Neutrophil Granulocyte Activation / Sipka Sándor, Keresztes Tamás, Kovács Ildikó, Sipka Sándor Jr., Baráth Sándor, Szegedi Gyula, Zeher Margit, Bruckner Geza
Dátum:2014
Megjegyzések:Objective: The objective was to investigate the early (20 minutes) effects of 21 L-amino acids on the activation of human neutrophils and to determine in healthy individuals the effects of a meal on the 1) number and relative luminescence unit (RLU) of peripheral neutrophils, 2) serum glutamate and glucose levels and 3) mTOR signaling network. Methods: The RLU of neutrophils stimulated by Ca2+ ionophor (CaI) and phorbol myristate acetate (PMA) following amino acid supplementation (3 x 10-4 M) or after consuming a meal was determined. L-glutamate was measured by HPLC. Results: All amino acids resulted in significant inhibitions of neutrophil RLU, except for arginine, which stimulated neutrophils. The ratios of amino acid induced inhibition were significantly higher in the cells stimulated by PMA than by CaI. The consumption of a meal resulted in a significant serum glutamate elevation compared to baseline (2.3 versus 0.9 x10-4 M) 90 minutes after ingestion of the meal. It was independent of the body mass index and returned near fasting levels after 150 minutes. The number of neutrophils was significantly elevated 90 minutes after the meal but the PMA induced RLU was significantly decreased. Conclusion: Our ex vivo and in vivo results suggest that the L-amino acids, independent of their metabolic significance, may continuosly and quickly modify the activity of human peripheral neutrophils, and also the outcome of various immunologic reactions. The activation of the mTORC1 complex likely involves a transient impairment in the function of mTORC2 complex in these processes.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
L-amino acids
L-glutamate
mTOR
neutrophils
RLU
Megjelenés:The Open Nutrition Journal. - 8 (2014), p. 1-7. -
További szerzők:Keresztes Tamás Kovács Ildikó Sipka Sándor ifj. (1980-) (orvos) Baráth Sándor (1977-) (biológus) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Zeher Margit (1957-2018) (belgyógyász, allergológus és klinikai immunológus, reumatológus) Bruckner Géza
Pályázati támogatás:71883
OTKA
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4.

001-es BibID:BIBFORM076597
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:A dietary amino acid load causes a transient decrease in the function of human neutrophil granulocytes / Sipka S., Bruckner G.
Dátum:2016
ISSN:0261-5614
Tárgyszavak:Orvostudományok Klinikai orvostudományok szerkesztői levél
Megjelenés:Clinical Nutrition. - 35 : 3 (2016), p. 770. -
További szerzők:Bruckner Géza
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5.

001-es BibID:BIBFORM041487
035-os BibID:PMID:18224288
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:Adenosine inhibits the release of arachidonic acid and its metabolites (AAM) in activated human peripheral mononuclear cells / S. Sipka, I. Kovács, S. Szántó, Gy. Szegedi, L. Brugós, G. Bruckner, A. J. Szentmiklósi
Dátum:2007
ISSN:1023-3830
Megjegyzések:The effects of adenosine (Ado) and subtype-specific activators of adenosine receptors (A(1), A(2A), A(2B) and A(3)) were studied on the release of arachidonic acid (AA) and its metabolites (AAM) from human peripheral mononuclear cells (monocytes). MATERIALS AND METHOD: Adenosine and the selective agonists and antagonists of adenosine receptors were used. (3)H-AA and its metabolites released into the medium were determined by measurement of the total (3)H radioactivity released without separating the AAM. RESULTS: In the cells activated by protein kinase C specific phorbol ester (phorbol 12-myristate 13-acetate) and Ca(2+) ionophore (A23187), adenosine and two subtype-specific receptor agonists, CPA(A(1)) and CGS 21680 (A(2A)) induced concentration-dependent inhibition of the release of AAM, whereas stimulation of A(2B) or A(3) receptors was ineffective. The rank order of potency for the inhibition of AAM release was as follows: CGS 21680 = CPA > adenosine > NECA (in the presence of ZM 24185 and DPCPX as A(2A) and A(1) adenosine receptor antagonists, respectively) = IB-MECA. Adenosine inhibited the release of AAM only at and above the concentration of 100 muM, whereas the inhibitory effect of A(1) and A(2A) receptor specific agonists appeared at a concentration of 10(-7) M. CONCLUSIONS: It can be concluded that adenosine physiologically may not have a significant effect on the AAM release of circulating monocytes, but in pathological conditions, where the local Ado concentrations increases, this nucleoside, through activation of A(2A) and A(1) receptors can exert, at least in part, an antiinflammatory action by decreasing proinflammatory AAM production.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
adenosine
arachidonic acid
Adenosine/pharmacology
Arachidonic Acid/metabolism
Calcimycin/pharmacology
Calcium/metabolism
Cyclic AMP/metabolism
Humans
Ionophores/pharmacology
Monocytes/drug effects/metabolism
Protein Kinase C/metabolism
Purinergic P1 Receptor Agonists
Purinergic P1 Receptor Antagonists
Tetradecanoylphorbol Acetate/pharmacology
egyetemen (Magyarországon) készült közlemény
Megjelenés:Inflammation Research 56 : 11 (2007), p. 468-472. -
További szerzők:Kovács I. (orvos) Szántó Sándor (1968-) (belgyógyász, reumatológus) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Brugós László (1951-) (tüdőgyógyász, klinikai immunológus, allergológus) Bruckner Géza Szentmiklósi József András (1948-) (farmakológus, klinikai laboratóriumi szakorvos)
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6.

001-es BibID:BIBFORM040669
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:Adenosine inhibits the release of interleukin-1beta in activated human peripheral mononuclear cells / Sandor Sipka, Ildikó Kovács, Sándor Szántó, Gyula Szegedi, László Brugós, Géza Bruckner, A. József Szentmiklósi
Dátum:2005
ISSN:1043-4666
Megjegyzések:The effects of adenosine and subtype-specific activators of adenosine receptors (A1, A2A, A2B and A3) were studied on the release of interleukin-1beta (IL-1beta) from peripheral mononuclear cells, monocytes and lymphocytes. In the cells activated by the protein kinase C specific phorbol ester (phorbol 12-myristate 13-acetate) and Ca(2+) ionophore (A23187) both adenosine and the subtype-specific receptor agonists, CPA (A1), CGS 21680 (A2A) and IB-MECA (A3) induced a concentration-dependent inhibition of IL-1beta release. The rank order of potency in the inhibition of IL-1beta release was CPA=CGS 21680>IB-MECA>adenosine>NECA (in the presence of A1, A2A and A3 receptor inhibitors). The inhibitory actions of CPA, CGS 21680 or IB-MECA were significantly reduced in the presence of DPCPX, ZM 243185 or MRS 1191 as subtype-specific antagonists on A1, A2A and A3 adenosine receptors, respectively. It can be concluded that adenosine inhibits the release of IL-1beta from the activated human peripheral mononuclear cells. In this process A1, A2A and A3 receptors are involved.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adenosine/analogs & derivatives/pharmacology
Calcimycin/pharmacology
Humans
Interleukin-1/blood
Monocytes/drug effects/metabolism
Phenethylamines/pharmacology
Purinergic P1 Receptor Agonists
Tetradecanoylphorbol Acetate/pharmacology
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytokine. - 31 : 4 (2005), p. 258-263. -
További szerzők:Kovács Ildikó Szántó Sándor (1968-) (belgyógyász, reumatológus) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Brugós László (1951-) (tüdőgyógyász, klinikai immunológus, allergológus) Bruckner Géza Szentmiklósi József András (1948-) (farmakológus, klinikai laboratóriumi szakorvos)
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