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001-es BibID:BIBFORM038646
035-os BibID:PMID:23109053
Első szerző:Gyimesi Edit (klinikai biokémikus, vegyész)
Cím:The effects of various doses of bacterial lipopolysaccharide on the expression of CD63 and the release of histamine by basophils of atopic and non-atopic patients / E. Gyimesi, F. Gönczi, M. Szilasi, G. Pál, S. Baráth, S. Sipka
Dátum:2013
ISSN:1023-3830
Megjegyzések:AbstractObjective We tested the effect of various doses of bacterial lipolysaccharide (LPS, endotoxin) on the expression of CD63 and the in vitro release of histamine by basophils stimulated with ragweed allergen in patients with or without ragweed and mite allergies. Methods The peripheral blood of 11 patients with ragweed allergy, 10 patients with mite allergy and 14 control patients was incubated with ragweed allergen extract following pretreatment with varying doses of LPS. The expression of CD63 in basophils was measured by flow cytometry, and the release of histamine was determined by ELISA. Results In the samples of patients with ragweed allergy that were exposed to specific allergen, only high doses of LPS significantly elevated the expression of CD63 (200 ng/ml; 1000 EU/ml) and the release of histamine (2000 ng/ml; 10000 EU/ml). There was no effect of LPS in any other cases. Conclusions Bacterial LPS (endotoxin) concentrations higher than 200 ng/ml (1000 EU/ml), which rarely occurs in nature, could only activate the basophils from atopic patients while in the presence of the specific allergen. Thus, the restoration of the urban, "microbe-poor" milieu with endotoxin (as LPS) can be a promising and harmless approach for allergy prevention.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
lipopolysaccharide
CD63
atopic
histamine
egyetemen (Magyarországon) készült közlemény
Megjelenés:Inflammation Research. - 62 : 2 (2013), p. 213-218. -
További szerzők:Gönczi Ferenc Szilasi Mária (1953-) (tüdőgyógyász, klinikai immunológus, allergológus, belgyógyász) Pál G. Baráth Sándor (1977-) (biológus) Sipka Sándor (1945-) (laboratóriumi szakorvos)
Internet cím:DOI
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