Találati lista | Tudóstér

001-es BibID:	BIBFORM019930
Első szerző:	Király Róbert (biológus)
Cím:	Coeliac autoantibodies can enhance transamidating and inhibit GTPase activity of tissue transglutaminase : dependence on reaction environment and enzyme fitness / Róbert Király, Zsófia Vecsei, Tamás Deményi, Ilma Rita Korponay-Szabó, László Fésüs
Dátum:	2006
ISSN:	0896-8411
Megjegyzések:	Modification of the enzymatic functions of tissue transglutaminase (TG2) by anti-TG2 autoantibodies may play a role in manifestations of coeliac disease. Our aim was to evaluate the effect of coeliac autoantibodies on reactions catalysed by TG2 by a systematic biochemical approach, and in relation to observed clinical presentation type. Coeliac antibodies did not have significant inhibitory effect on transamidation/deamidation activity of TG2 as measured by amine-incorporation into solid and immobilised casein and by ultraviolet kinetic assay. In contrast, immunoglobulins from patients with severe malabsorption enhanced the reaction velocity to 105.4-242.2%. This activating effect was dose-dependent, most pronounced with immobilised glutamine-acceptor substrates, and correlated inversely with the basal specific activity of the enzyme and with dietary treatment. A similar activation could be demonstrated also with the TG2-specific fraction of autoantibodies and in transamidation activity assays which use fibronectin-bound TG2 and thereby mimic in vivo conditions. These results suggest that coeliac antibodies may stabilise the enzyme in a catalytically advantageous conformation. GTPase activity of TG2 decreased to 67.0-73.4% in the presence of antibodies raising the possibility that inhibition of GTPase activity may affect cellular signalling in case coeliac autoantibodies would reach intracellular compartments.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Autoimmunity 26 : 4 (2006), p. 278-287. -
További szerzők:	Simon-Vecsei Zsófia (1980-) (biológus) Deményi Tamás Korponay-Szabó Ilma (1959-) (gyermekgyógyász) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	TS044798 OTKA T043083 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: