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001-es BibID:	BIBFORM049594
035-os BibID:	PMID:23846866
Első szerző:	Doan-Xuan, Quang-Minh (biofizikus)
Cím:	High content analysis of differentiation and cell death in human adipocytes / Quang Minh Doan-Xuan, Anitta K. Sarvari, Pamela Fischer-Posovszky, Martin Wabitsch, Zoltan Balajthy, Laszlo Fesus, Zsolt Bacso
Dátum:	2013
ISSN:	1552-4922 1552-4930
Megjegyzések:	Understanding adipocyte biology and its homeostasis is in the focus of current obesity research. We aimed to introduce a high-content analysis procedure for directly visualizing and quantifying adipogenesis and adipoapoptosis by laser scanning cytometry (LSC) in a large population of cell. Slide-based image cytometry and image processing algorithms were used and optimized for high-throughput analysis of differentiating cells and apoptotic processes in cell culture at high confluence. Both preadipocytes and adipocytes were simultaneously scrutinized for lipid accumulation, texture properties, nuclear condensation, and DNA fragmentation. Adipocyte commitment was found after incubation in adipogenic medium for 3 days identified by lipid droplet formation and increased light absorption, while terminal differentiation of adipocytes occurred throughout day 9-14 with characteristic nuclear shrinkage, eccentric nuclei localization, chromatin condensation, and massive lipid deposition. Preadipocytes were shown to be more prone to tumor necrosis factor alpha (TNFα)-induced apoptosis compared to mature adipocytes. Importantly, spontaneous DNA fragmentation was observed at early stage when adipocyte commitment occurs. This DNA damage was independent from either spontaneous or induced apoptosis and probably was part of the differentiation program.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban DNA damage SGBS adipocyte differentiation high content analysis laser scanning cytometry preadipocyte Doktori iskola
Megjelenés:	Cytometry Part A. - 83 : 10 (2013), p. 933-943. -
További szerzők:	Sárvári Anitta Kinga (1984-) (biológus) Fischer-Posovszky Pamela Wabitsch, Martin Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus) Bacsó Zsolt (1963-) (biofizikus)
Pályázati támogatás:	NK 105046 OTKA OMFB-01626/2006 OTKA GVOP-3.2.1-2004-04-0351/3.0 GVOP TECH09-A2-2009-0131 GVOP TRANSCOM IAPP 251506 FP7 NKTH NTP Schizo08 Egyéb TÁMOP-4.2.2/B-10/1-2010-0024 TÁMOP Molekuláris Sejt- és Immunbiológiai Doktori Iskola TÁMOP-4.2.2.A-11/1/KONV-2012-0023-"VÉD-ELEM" TÁMOP
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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