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001-es BibID:BIBFORM055584
035-os BibID:Article ID: P-008
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Sperm fertilization potential and chromosomal anomalies at decreased sperm concentration : differences between testicular cancer patients and infertile men / A. Jakab, A. Mokánszki, A. Varga, M. Benyó, Z. Kassai, E. Oláh, Z. Molnár
Dátum:2013
ISSN:0268-1161
Megjegyzések:Study questionTo compare the sperm hyaluronic acid (HA) binding capacity and aneuploidy frequency of testicular germ cell carcinoma (TGCC) patients and infertile men without testicular cancer, but with decreased sperm concentrations.Summary answerWhile decreased fertilization potential reflected by low HA binding are related to low sperm count in both groups, elevated frequencies of chromosomal abnormalities are detected only in infertile, but not in TGCC patients. Qualitative spermiogenesis seems to be dissimilarly defected in TGCC and in infertile men without testicular cancer.What is known alreadyQuantitative spermatogenesis is known to be defected in men with TGCC, but little is known about the sperm quality and whether oligozoospermia arises from testicular alteration due the surgery or the tumor. Cryopreservation is the standard choice to preserve fertility. Infertile men with low sperm concentrations have higher frequency of aneuploid spermatozoa, which can be transmitted with assisted reproduction. HA binding capacity related to sperm maturity, viability, normal chromatin structure and fertilization potential.Study design, size, durationThis analysis is part of a prospective cohort study recruiting TGCC patients referred for cryopreservation and infertile men referred for genetic and functional sperm analysis in an academic tertiary center from 2010 to 2012. In this analysis patients with sperm count <40M/mL were included before treatment.Participants/materials, setting, methodsTwenty-one TGCC patients and 26 infertile men were examined. Spermiogram, Hyaluronan Binding Assay (HBA) was performed on native samples, HBA scores (%Bound = 100xBound Motile/Total Motile) were calculated. Sperm aneuploidy and diploidy frequency were examined with triple-color FISH scoring ca.5,000 spermatozoa of each individuals, then estimated numerical chromosome aberrations were calculated.Main results and the role of chanceMean sperm concentration was slightly higher (18.7 M/mL, range 6-35) in TGCC patients (mean age 28.7, range 18-37) than in infertile men (12.3 M/mL, range 1-39, mean age 34.4, 27-42) (p < 0.05). HBA score was 54.1% (range 8-86.5) in TGCC patients and even lower, 38.7% (0-83) in the infertile group (p < 0.05). The X/Y ratio were close to 1:1 in both groups. Estimated numerical chromosome aberrations were higher in the infertile than in the TGCC group (6.08% vs. 4.12%, p < 0.001), with elevations of both autosomal aneuploidies (0.11% vs. 0.07% of disomies 17, p < 0.001) and diploidies (0.30% vs. 0.15%, p < 0.001), but not sex chromosome disomies (0.41% vs. 0.40%, p = 0.1628). Samples normality was analysed using Shapiro-Wilk test, homogenity with Barlett test and the significance level with Two-sample t-probe.Limitations, reason for cautionMean sperm concentration is higher in TGCC patients than in the infertile group, since considering the limited amount for cryopreservation only samples < 6 M/mL were used for study purposes in those men.Wider implications of the findingsLow fertilization potential reflected in decreased sperm count and low hyaluronic binding capacity occurs in both TGCC and infertile patients. However, while the sperm aneuploidy in increased in infertile men, TGCC patients show similar rate to normal population. The presence of cancer does not increase the frequency of sperm numerical chromosomal aberrations. Thus, unlike in oligozoospermic infertile men, semen cryopreservation and assisted reproduction in TGCC do not increase risk of transmitting major genetic defects.Study funding/competing interest(s)Study was founded by the participating institutions and by the PhD scholarships. No competing interest declared.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm
testicular cancer
infertility
aneuploidy
hyaluronic binding capacity
Megjelenés:Human Reproduction. - 28 : S1 (2013), [2] p. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Varga Attila (1949-) (urológus, andrológus) Benyó Mátyás (1982-) (urológus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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