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001-es BibID:BIBFORM061085
035-os BibID:(WoS)000366646400030 (Scopus)84951573719
Első szerző:Ujj Zsófia
Cím:WT1 Expression in Adult Acute Myeloid Leukemia : assessing its Presence, Magnitude and Temporal Changes as Prognostic Factors / Zsófia Ujj, Gergely Buglyó, Miklós Udvardy, Dániel Beyer, György Vargha, Sándor Biró, László Rejtő
Dátum:2016
ISSN:1219-4956 1532-2807
Megjegyzések:Expression of the gene Wilms tumor 1 (WT1) has been suggested as a marker of minimal residual disease in acute myeloid leukemia (AML), but literature data are not without controversy. Our aimwas to assess the presence, magnitude and temporal changes ofWT1 expression as prognostic factors. 60 AML patients were followed until death or the end of the 6-year observation period. Blood samples were taken at diagnosis, post-induction, during remission and in case of a relapse. Using quantitative real-time PCR, we determined WT1 expression from each sample, normalized it against the endogenous control gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and classified samples as negative, moderately positive or highly positive. We divided the patients into groups based on detected WT1 expression values, illustrated overall and disease-free survival on Kaplan-Meier curves, and compared differences between each group by the logrank test. Disappearance of WT1-positivity during chemotherapy had a favorable effect on survival. Interestingly, no difference was seen between the survivals of WT1-positive subgroups that expressed moderate or high levels of WT1 mRNA. A 1-log decrease in WT1 expression without becoming negative did not affect prognosis, either. Our results suggest that defining a cut-off value for WT1-positivity, rather than just using logarithmic figures of changes in gene expression, might have prognostic use in post-induction AML patients. We encourage further, larger-scale studies.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
WT1 gene
Overexpression
Quantitative
real-time PCR
Acute myeloid leukemia
Megjelenés:Pathology & Oncology Research. - 22 : 1 (2016), p. 217-221. -
További szerzők:Buglyó Gergely (1980-) (genetikus) Udvardy Miklós (1947-) (belgyógyász, haematológus) Beyer Dániel (1982-) (molekuláris biológus) Vargha György (1951-) (orvos) Biró Sándor (1949-) (molekuláris genetikus) Rejtő László (1963-) (belgyógyász, haematológus)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0025
TÁMOP
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