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001-es BibID:BIBFORM062076
Első szerző:Csősz Éva (biokémikus, molekuláris biológus)
Cím:Highly abundant defense proteins in human sweat as revealed by targeted proteomics and label-free quantification mass spectrometry / É. Csősz, G. Emri, G. Kallo, G. Tsaprailis, J. Tőzsér
Megjegyzések:Background The healthy human skin with its effective antimicrobial defense system forms an efficient barrier againstinvading pathogens. There is evidence suggesting that the composition of this chemical barrier varies between diseases,making the easily collected sweat an ideal candidate for biomarker discoveries.Objective Our aim was to provide information about the normal composition of the sweat, and to study the chemicalbarrier found at the surface of skin.Methods Sweat samples from healthy individuals were collected during sauna bathing, and the global protein panelwas analysed by label-free mass spectrometry. SRM-based targeted proteomic methods were designed and stableisotope labelled reference peptides were used for method validation.Results Ninety-five sweat proteins were identified, 20 of them were novel proteins. It was shown that dermcidin is themost abundant sweat protein, and along with apolipoprotein D, clusterin, prolactin-inducible protein and serum albumin,they make up 91% of secreted sweat proteins. The roles of these highly abundant proteins were reviewed; all of whichhave protective functions, highlighting the importance of sweat glands in composing the first line of innate immunedefense system, and maintaining the epidermal barrier integrity.Conclusion Our findings with regard to the proteins forming the chemical barrier of the skin as determined by label-freequantification and targeted proteomics methods are in accordance with previous studies, and can be further used as astarting point for non-invasive sweat biomarker research.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
epidermal barrier
highly abundant sweat proteins
label free quantification
non-immune defense
targeted proteomics
Megjelenés:Journal Of The European Academy Of Dermatology And Venereology. - 29 : 10 (2015), p. 2024-2031. -
További szerzők:Emri Gabriella (1972-) (bőrgyógyász, allergológus, onkológus) Kalló Gergő (1989-) (molekuláris biológus) Tsaprailis, George Tőzsér József (1959-) (molekuláris biológus, biokémikus, vegyész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
Proteomika, Szemészet Kutatócsoport
T AMOP-4.2.4.-A/2-11-1-2012-0001
Training Grant Number ? ES007091
NIEHS grant ES06694
NIH/NCI grant CA023074
NIH/NCRR 1S10 RR028868-01
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