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001-es BibID:	BIBFORM065027
Első szerző:	Kovács Csilla (élelmiszerbiztonsági és minőségi mérnök)
Cím:	Endophytic and pathogenic fungi associated with Grapevine Trunk Diseases in the Tokaj wine region, Hungary : In: Abstracts of oral and poster presentations given at the 9th International Workshop on Grapevine Trunk Diseases, Adelaide, Australia, 18-20 November 2014 / Cs. Kovács, E. Sándor, Z. Bihari, F. Peles
Dátum:	2014
ISSN:	0031-9465 1593-2095
Megjegyzések:	Grapevine trunk diseases (GTD) are amongst the most important diseases in vineyards worldwide. GTD are caused by several pathogenic fungi including, Phaeoacremonium aleophilum, Phaeomoniella chlamydospora, Eutypa lata, Fomitiporia mediterranea, Diplodia seriata among many others. However, there is currently very limited information about the status of GTD pathogens in the Tokaj wine region in Hungary. In this study, symptomatic vine samples showing GTD foliar symptoms were collected from vineyards in four areas in the Tokaj region in 2013: Szemere (Degenfeld); Dorgó (Disznókő); Szarvas (Kereskedőház), and Várhegy (Patrícius) The woody tissue samples were cut in a small pieces, and were disinfected before plating on malt extract agar and incubated at room temperature for 3-8 days. Pathogenic fungi were isolated from 207 symptomatic grape trunks. DNA isolation from fungal colonies was conducted with the NucleoSpin Plant II kit. The ITS4 and ITS5 primers were used for PCR amplification of the ITS1-5.8S-ITS2 region. Purified amplicons were sequenced by Mycrosinth, Austria. Consensus sequences were aligned with reference sequences using Clustal X and manually corrected with GeneDoc. Phylogenetic analyses were performed with MEGA 5.05. To date, 366 pure fungal cultures were isolated and identified. The majority of the isolates (56%) were determined as Diplodia seriata (Botryosphaeriaceae). Other fungi, like Fusarium sp. (13.9%), Alternaria sp. (9.3%), Aspergillus sp. (7.9%), Mucor sp. (7.1%), Diaporthe sp. (3.3%), Trichoderma sp. (1.4%), Epicoccum sp. (0.5%), Penicillium (0.3%) and Xanthomendoza sp. (0.3%) were also identified from grapevine trunk samples.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idézhető absztrakt Grapevine Trunk Diseases Tokaj wine region
Megjelenés:	Phytopathologia Mediterranea. - 53 : 3 (2014), p. 566. -
További szerzők:	Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Bihari Zoltán (1967-) (biológus) Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
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