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001-es BibID:	BIBFORM065077
Első szerző:	Peles Ferenc (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Cím:	Species identification of wine cellar mould derived from the Tokaj wine region, Hungary / Ferenc Peles, Csilla Kovács, Anikó Szojka, Zoltán Bihari, Erzsébet Sándor
Dátum:	2015
ISSN:	1217-8950 1588-2640
Megjegyzések:	Zasmidium cellare (wine cellar mould, Syn.: Cladosporium cellare) was reported previously the most frequently occurred mould on the inner surfaces of the wine cellars , but other mould genus (e.g. Alternaria, Aspergillus, Aureobasidium, Cladosporium, Geosmithia, Penicillium, and Verticillium) have been identified, too. Since very few reports have been published in this topic in Hungary, we aimed to identify some isolates derived from the inner surface of some winery from the Tokaj wine region. Mycelial samples were collected from the inner surface of two cellars near to Tokaj in 2014 spring. The samples were collected with sterile forceps and were transported in sterile centrifuge tube to the Microbiological Laboratory of Institute of Food Science (University of Debrecen). Sample preparation and microbiological examination were carried out according to the protocol of Goto et al. (1989). Following the morphological tests, the molecular biological identification of the isolates was performed with the help of ITS1 and ITS2 sequences (Schoch et al., 2012). ITS4 and ITS5 primers were used for PCR amplification of the internal transcribed spacer regions (White et al., 1990). The PCR products were purified with using NucleoSpin Gel and PCR Clean-up. Purified amplicons were sequenced by Mycrosinth, Austria. The sequences were aligned with Clustal X program, and manually corrected with GeneDoc. Phylogenetic analyses were performed with MEGA 5.05.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idézhető absztrakt wine cellar mould Tokaj wine region
Megjelenés:	Abstracts of the Annual Meeting of the Hungarian Society for Microbiology and EU FP-7 PROMISE Regional Meeting. Acta Microbiologica et Immunologica Hungarica. - 62 : Suppl. 1 (2015), p. 88. -
További szerzők:	Kovács Csilla (1990-) (élelmiszerbiztonsági és minőségi mérnök) Bérczesné Szojka Anikó (1987-) (biomérnök) Bihari Zoltán (1967-) (biológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
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