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001-es BibID:	BIBFORM076044
035-os BibID:	(WoS)000436909600011 (Scopus)85054071643 (PubMed)29769426
Első szerző:	Horváth Balázs (élettanász)
Cím:	Effect of the intracellular calcium concentration chelator BAPTA acetoxy-methylester on action potential duration in canine ventricular myocytes / B. Horvath, N. Szentandrassy, R. Veress, D. Baranyai, K. Kistamas, J. Almassy, A. Toth, J. Magyar, T. Banyasz, P. P. Nanasi
Dátum:	2018
Megjegyzések:	Intracellular calcium concentration ([Ca2+]i) is often buffered by using the cell-permeant acetoxy-methylester form of the Ca2+ chelator BAPTA (BAPTA-AM) under experimental conditions. This study was designed to investigate the time-dependent actions of extracellularly applied BAPTA-AM on action potential duration (APD) in cardiac cells. Action potentials were recorded from enzymatically isolated canine ventricular myocytes with conventional sharp microelectrodes. The effect of BAPTA-AM on the rapid delayed rectifier K+ current (IKr) was studied using conventional voltage clamp and action potential voltage clamp techniques. APD was lengthened by 5 ?M BAPTA-AM - but not by BAPTA - and shortened by the Ca2+ ionophore A23187 in a time-dependent manner. The APD-lengthening effect of BAPTA-AM was strongly suppressed in the presence of nisoldipine, and enhanced in the presence of BAY K8644, suggesting that a shift in the [Ca2+]i-dependent inactivation of L-type Ca2+ current may be an important underlying mechanism. However, in the presence of the IKr-blocker dofetilide or E-4031 APD was shortened rather than lengthened by BAPTA-AM. Similarly, the APD-lengthening effect of 100 nM dofetilide was halved by the pretreatment with BAPTA-AM. In line with these results, IKr was significantly reduced by extracellularly applied BAPTA-AM under both conventional voltage clamp and action potential voltage clamp conditions. This inhibition of IKr was partially reversible and was not related to the Ca2+ chelator effect BAPTA-AM. The possible mechanisms involved in the APD-modifying effects of BAPTA-AM are discussed. It is concluded that BAPTA-AM has to be applied carefully to control [Ca2+]i in whole cell systems because of its direct inhibitory action on IKr.
Tárgyszavak:	idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk calcium chelators intracellular calcium concentration action potential duration cardiac ion currents potassium ion currents ventricular myocytes
Megjelenés:	Journal of Physiology and Pharmacology. - 69 : 1 (2018), p. 99-107. -
További szerzők:	Szentandrássy Norbert (1976-) (élettanász) Veress Roland (1992-) (molekuláris biológus) Baranyai Dóra Kistamás Kornél (1986-) (biológus) Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító) Tóth A. (farmakológus) Magyar János (1961-) (élettanász) Bányász Tamás (1960-) (élettanász) Nánási Péter Pál (1956-) (élettanász)
Pályázati támogatás:	NKFIHK-115397 NKFIH NKFIH-K109736 NKFIH NKFIH-PD120794 NKFIH OTKA ANN-113273 OTKA GINOP-2.3.2-15-2016-00040 GINOP EFOP-3.6.2-16-2017-00006 EFOP UNKP-17-4-III-DE-201 UNKP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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