Találati lista | Tudóstér

001-es BibID:	BIBFORM077496
Első szerző:	Saravanabhavan, Shanmuga Sundar
Cím:	Graphene oxide functionalized with chitosan based nanoparticles as a carrier of siRNA in regulating Bcl-2 expression on Saos-2 & MG-63 cancer cells and its inflammatory response on bone marrow derived cells from mice / Shanmuga Sundar Saravanabhavan, Muruganantham Rethinasabapathy, Sarang Zsolt, Aravind Bhat Kalambettu, Sundaravadivel Elumalai, Manokaran Janakiraman, Huh Yun Suk, Balasubramanian Natesan
Dátum:	2019
ISSN:	0928-4931
Megjegyzések:	Presently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nanoparticles were loaded with siRNA, studied for in vitro release with varying concentration & pH, and fitted to peppas model. MTT & ROS assay was used to evaluate biocompatibility of carrier and qPCR to study the inflammatory responses in particular checking gene expression of IL-6, TGF-?, TNF-? in both RAW 264.7 and bone marrow derived macrophages. The results of study showed that release of siRNA were in a controlled fashion and effective at acidic pH that prevails on tumor site. The material was biocompatible and effective in case of Saos-2 osteosarcoma cells with a viability of 0.4?0.43 and 0.49?0.53 in case of MG-63 cells when treated with highest concentration of 100?l siRNA compared to untreated cells that were in range of 0.64?0.67 in Saos-2 and 0.61?0.63 in MG-63 cells. The results of expression of inflammatory cytokines IL-6, TGF-? & TNF-? showed negligible amount compared to control group serving the purpose of an effective carrier targeting tumor cells.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Materials Science & Engineering C-Biomimetic And Supramolecular Systems. - 99 (2019), p. 1459-1468. -
További szerzők:	Rethinasabapathy, Muruganantham Sarang Zsolt (1976-) (mikrobiológus) Kalambettu, Aravind Bhat Elumalai, Sundaravadivel Janakiraman, Manokaran Huh, Yun Suk Natesan, Balasubramanian
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: