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001-es BibID:BIBFORM101791
035-os BibID:(cikkazonosító)82
Első szerző:Khangembam, Rojesh
Cím:Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples / Rojesh Khangembam, Mariann Tóth, Nóra Vass, Marián Várady, Levente Czegléd1, Róbert Farkas, Alistair Antonopoulos
Dátum:2021
ISSN:1776-1042
Megjegyzések:In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Ko?sice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main ratelimiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC technique were also utilised, which demonstrated that it could be efficiently adapted for field-level egg concentration to extract DNA. This proof of concept study showed that isothermal amplification technologies with a colourimetric detection or when combined with a lateral flow assay could be an important step for a true point of care molecular diagnostic assay for H. contortus.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
LAMP assay
Lateral flow
Haemonchus contortus
Point-of-Care Diagnosis
Fill-FLOTAC
Megjelenés:Parasite. - 28 (2021), p. 1-12. -
További szerzők:Tóth Mariann (1994-) (agrármérnök) Vass Nóra (1983-) (állatorvos) Várady Marián Czeglédi Levente (1977-) (agrármérnök) Farkas Róbert Antonopoulos, Alistair
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00008
EFOP
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