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001-es BibID:BIBFORM104955
Első szerző:Bakos Zsuzsanna
Cím:Evaluation of molecular biological markers of different sweet corn (Zea mays L. convar. saccharata Koern) genotypes / Bakos Zsuzsanna, Nagy János, Parádi István, Demeter Cintia, Szűcs Kata, Szántó-Egész Réka, Micsinai Adrienn
Megjegyzések:Sweet corn (Zea mays L. convar saccharata Koern) is a popular vegetable all over the world. In the following years it is expected that research will focus on these varieties due to their important dietary role. Hungary has an outstanding record in the cultivation of sweet corn, being the largest producer in Europe and the world's second largest exporter - an average annual production of 500 thousand tons, 95% of which is exported. Plant carotenoids have been implicated in preventing several age-related diseases. Numerous studies have identified lutein and zeaxanthin to be essential components for eye health. They have also been linked with reduced risk of age-related macular degeneration (AMD) and cataracts, thus increasing the content of carotenoids in maize grains is of great interest. In our study we aim to follow the molecular biological markers of lutein biosynthesis by targeted gene expression studies in three super sweet maize varieties: Dessert R78, Messenger and Honey during the generative phase of the plants. At first, reliable reference genes will be identified for data normalization. Samples were collected from the topmost developed leaves of the plants, frozen immediately in liquid nitrogen and transported to the laboratory. RNA isolation included homogenization under liquid nitrogen, followed by RNA isolation, then it was transcribed to cDNA by random hexamer method. Real-time PCR was carried out on a Thermo QuantStudio 5 device utilizing SYBR dye kits. Previously we selected four reference genes for our studies, which encode tubulin (TUB), ubiquitin (UBI), actin (ACT) and a thioredoxin-like gene (TLG). These housekeeping genes consist of a group of constitutively expressed genes which are considered to be essential to maintain basic cellular functions, and are ubiquitously expressed in all cells of an organism, irrespective of tissue type, developmental stage, cell cycle state, or external signal. From these four candidate reference genes three (TUB, UBI and ACT) gave satisfactory results and selected for further downstream studies. The PCR products have also been confirmed by sequencing and sequence alignment to respective genes. For lutein biosynthesis gene expression, 7 target genes and 8 pairs of primers were selected for preliminary studies (PSY, HYD, CYP97C, PDS, ZDS, LCYB, LCYE). These indicated that all real-time PCR reactions are suitable for further studies in our selection of sweet corn varieties
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok előadáskivonat
Megjelenés:Scientific Conference of PhD Students of FAFR, FBFS and FHLE SUA in Nitra with international participation : Proceedings of abstracts / eds. Monika Tóthová, Judita Lidiková, Kristína Candráková, Dominik Hollý. - p. 65-65. -
További szerzők:Nagy János (1951-) (agrármérnök, mérnök-tanár) Parádi István Demeter Cintia (1992-) (agrármérnök, növénytermesztő) Szűcs Kata Szántó-Egész Réka Micsinai Adrienn (1972-) (biológus, mikrobiológus)
Pályázati támogatás:TKP2020-IKA-04
Internet cím:DOI
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