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001-es BibID:	BIBFORM125048
035-os BibID:	(scopus)85206405789 (wos)001342478500018
Első szerző:	Patsalos, Andreas
Cím:	Spatiotemporal transcriptomic mapping of regenerative inflammation in skeletal muscle reveals a dynamic multilayered tissue architecture / Patsalos Andreas, Halasz Laszlo, Oleksak Darby, Wei Xiaoyan, Nagy Gergely, Tzerpos Petros, Conrad Thomas, Hammers David W., Sweeney H. Lee, Nagy Laszlo
Dátum:	2024
ISSN:	0021-9738 1558-8238
Megjegyzések:	Tissue regeneration is orchestrated by macrophages that clear damaged cells and promote regenerative inflammation. How macrophages spatially adapt and diversify their functions to support the architectural requirements of actively regenerating tissue remains unknown. In this study, we reconstructed the dynamic trajectories of myeloid cells isolated from acutely injured and early stage dystrophic muscles. We identified divergent subsets of monocytes/macrophages and DCs and validated markers (e.g., glycoprotein NMB [GPNMB]) and transcriptional regulators associated with defined functional states. In dystrophic muscle, specialized repair-associated subsets exhibited distinct macrophage diversity and reduced DC heterogeneity. Integrating spatial transcriptomics analyses with immunofluorescence uncovered the ordered distribution of subpopulations and multilayered regenerative inflammation zones (RIZs) where distinct macrophage subsets are organized in functional zones around damaged myofibers supporting all phases of regeneration. Importantly, intermittent glucocorticoid treatment disrupted the RIZs. Our findings suggest that macrophage subtypes mediated the development of the highly ordered architecture of regenerative tissues, unveiling the principles of the structured yet dynamic nature of regenerative inflammation supporting effective tissue repair.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Expression profiling Inflammation Macrophages Skeletal muscle
Megjelenés:	Journal Of Clinical Investigation. - 134 : 20 (2024), p. 1-20. -
További szerzők:	Halász László (1989-) (molekuláris biológus) Oleksak, Darby Wei, Xiaoyan Nagy Gergely (1986-) (molekuláris biológus) Tzerpos, Petros (1982-) (molekuláris biológus) Conrad, Thomas Hammers, David W. Sweeney, H. Lee Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Pályázati támogatás:	KKP129909 OTKA FK146945 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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