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001-es BibID:	BIBFORM125049
035-os BibID:	(scopus)85207447988 (wos)001341579100001
Első szerző:	Guba Andrea (Okleveles vegyész)
Cím:	Analysis of Azathioprine Metabolites in Autoimmune Hepatitis Patient Blood-Method Development and Validation / Guba Andrea, Kováts Patrícia, Mezei Zoltán A., Papp Mária, Csősz Éva, Kalló Gergő
Dátum:	2024
ISSN:	1661-6596 1422-0067
Megjegyzések:	Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease treated by steroids and immunomodulator thiopurine drugs such as azathioprine (AZA). AZA is metabolized in the human body into bioactive forms such as 6-thioguanine (6-TG) and 6-methyl-mercaptopurine (6-MMP). Monitoring the levels of bioactive AZA metabolites is very important for proper treatment of patients. In this study, our aim was to develop and validate a fast and sensitive ultra-high performance liquid chromatography?mass spectrometry (UHPLC?MS) method for the analysis of 6-TG and 6-MMP from blood samples of patients with AIH to monitor the level of these bioactive metabolites. The detection and quantification of the analytes was carried out by Selected Reaction Monitoring (SRM)-based targeted mass spectrometry. The method was validated according to the EMA guidelines. Blood samples from patients with AIH treated with AZA were analysed with the developed method. The method was successfully validated with appropriate accuracy and precision for the target biomolecules and their concentration in the samples from patients with AIH was determined. The developed and validated UHPLC?MS method enables the fast and precise analysis of AZA metabolites.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	International Journal Of Molecular Sciences. - 25 : 20 (2024), p. 1-12. -
További szerzők:	Kováts Patrícia (1995-) Mezei Zoltán András (1980-) (orvos) Papp Mária (1975-) (belgyógyász, gasztroenterológus) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Kalló Gergő (1989-) (molekuláris biológus)
Pályázati támogatás:	GINOP-2.3.3-15-2016-00020 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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