Találati lista | Tudóstér

001-es BibID:	BIBFORM069263
Első szerző:	Szebeni János
Cím:	Plasma Proteome Profiling with Monoclonal Antibody Libraries : a Pilot Biomarker Analysis for Nanomedicine-Induced Complement Activation / Szebeni János, Weiszhár Zsóka, Rozsnyay Zoltán, Martinsky Todd, Kádas János, Lázár József, Takács László, Kurucz István
Dátum:	2013
ISSN:	2169-0510 2169-0529
Megjegyzések:	Complement (C) activation-related hypersensitivity reactions (HSRs) represent an unsolved adverse immune effect of many i.v. administered "nanomedicines", such as liposomal doxorubicin (Doxil/Caelyx). Because these pseudoallergic reactions can be severe or even lethal, it is an important clinical objective to find biomarkers for proneness for C activa-tion by reactogenic nanoparticles that will allow the prediction of in vivo reactions by in vitro assays. With this goal in mind we identified a normal human blood donor whose serum consistently showed high sensitivity to Caelyx-induced C activation in vitro (CSS). The plasma of this blood (Caelyx-sensitive plasma, CSP) was subjected to proteome profiling with a library of human plasma proteome specific mAbs. The chip (PlasmaScan-380TM) contained 380 non-redundant (with respect to epitopes) mAbs. The analysis revealed 8 proteins that were differentially represented in CSP in com-parison with Caelyx-insensitive control plasma. These proteins were identified by mass spectrometry and Western blot analyses to represent factor H (decreased in CSP), factor H related protein, serum amyloid P component, fibronectin, complement component C4, Apo B100, prothrombin and alpha-2-HS glycoprotein (all increased in CSP). Some of these protein changes are consistent with proneness for increased C activation, suggesting the potential use of this method in the search for biomarkers for liposome-induced or other types of nanomedicine-induced HSRs.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Hypersensitivity Reactions Complement Factor H Serum Amyloid P Proteome Profiling Biomarkers
Megjelenés:	Advances in Nanoparticles. - 2 : 2 (2013), p. 133-144. -
További szerzők:	Weiszhár Zsóka Rozsnyay Zoltán Martinsky, Todd Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Lázár József Takács László (1955-) Kurucz István
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: