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001-es BibID:	BIBFORM045835
Első szerző:	Kurzakowski, W.
Cím:	Radioactive penicillin G production by immobilized fungal vesicles / W. Kurzakowski, W. Kuryłowicz, A. Penyige
Dátum:	1984
ISSN:	0175-7598
Megjegyzések:	Radioactive penicillin G production from l-[1-14C]-valine (1.75 GBq ? mmol-1) by native and by calcium alginate gel immobilized mycelium of Penicillium chrysogenum PQ-96 in a medium for antibiotic production as well as by vesicles isolated from the protoplasts of the same strain in a well-defined reaction mixture was investigated. Specific radioactivity of the penicillin G produced by the native vesicles was 1.45 GBq ? mmol-1 and that of the antibiotic synthesized by the calcium alginate gel immobilized vesicles was 1.48 GBq ? mmol-1. By comparison, the specific radioactivity of penicillin G produced by native mycelium was 0.42 GBq ? mmol-1 and of that synthesized by the immobilized mycelium was 0.96 GBq ? mmol-1. Production of radioactive penicillin G by native and immobilized vesicles in repeated use was also investigated. At the beginning of the production phase, the radioactive penicillin G synthesized by the immobilized vesicles was 25 nmol ? mg protein-1 ? h-1 and decreased after 8 days to a level of 11 nmol ? mg protein-1 ? h-1. The half-life of the immobilized vesicles was 7 days. The native vesicles showed a rapid decrease in radioactive antibiotic production. In comparison, the penicillin G production in a repeated use of immobilized vesicles decreased during 40 days from 140 nmol ? mg protein-1 ? h-1 to 60 nmol ? mg protein-1 ? h-1. The half-life of the immobilized vesicles was 35 days. The native vesicles showed after 4 days a lack of activity of penicillin G production. The stability of immobilized mycelium or vesicles in the process of radioactive penicillin G production is discussed.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Penicillium chrysogenum penicillin G production
Megjelenés:	Applied Microbiology and Biotechnology. - 19 : 5 (1984), p. 312-315. -
További szerzők:	Kuryłowicz, W. Penyige András (1954-) (molekuláris genetikus)
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