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001-es BibID:	BIBFORM069259
Első szerző:	Hajdú István (Dunakeszi)
Cím:	Monoclonal antibody proteomics : use of antibody mimotope displaying phages and the relevant synthetic peptides for mAb scouting / Hajdú István, Flachner Beáta, Bognár Melinda, Végh Barbara M., Dobi Krisztina, Lőrincz Zsolt, Lázár József, Cseh Sándor, Takács László, Kurucz István
Dátum:	2014
ISSN:	0165-2478
Megjegyzések:	Monoclonal antibody proteomics uses nascent libraries or cloned (PlasmascanTM, QuantiPlasmaTM)libraries of mAbs that react with individual epitopes of proteins in the human plasma. At the initialphase of library creation, cognate protein antigen and the epitope interacting with the antibodies arenot known. Scouting for monoclonal antibodies (mAbs) with the best binding characteristics is of highimportance for mAb based biomarker assay development. However, in the absence of the identity ofthe cognate antigen the task represents a challenge. We combined phage display, and surface plasmonresonance (Biacore) experiments to test whether specific phages and the respective mimotope peptidesobtained from large scale studies are applicable to determine key features of antibodies for scouting. Weshow here that mAb captured phage-mimotope heterogeneity that is the diversity of the selected peptidesequences, is inversely correlated with an important binding descriptor; the off-rate of the antibodies andthat represents clues for driving the selection of useful mAbs for biomarker assay development. Carefullychosen synthetic mimotope peptides are suitable for specificity testing in competitive assays using thetarget proteome, in our case the human plasma.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Proteomics Monoclonal antibod y Phage-display Surface plasmon resonance Affinity
Megjelenés:	Immunology Letters. - 160 : 2 (2014), p. 172-177. -
További szerzők:	Flachner Beáta Bognár Melinda Végh Barbara M. Dobi Krisztina Lőrincz Zsolt (1979-) (Biológus) Lázár József Cseh Sándor (1954-) (állatorvos) Takács László (1955-) Kurucz István
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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