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001-es BibID:	BIBFORM065593
Első szerző:	Gall-Debreceni Anna
Cím:	Specific detection and quantitation of bovine IgG in bioreactor derived mouse mAb preparations / Anna Gall-Debreceni, Jozsef Lazar, Janos Kadas, Attila Balogh, Annamaria Ferenczi, Endre Sos, Laszlo Takacs, Istvan Kurucz
Dátum:	2016
ISSN:	0022-1759
Megjegyzések:	Monoclonal antibody and recombinant protein production benefits greatly from bovine serum as an additive. The caveat is that bovine serum IgG, co-purifies with mAbs and IgG Fc-containing fusion proteins and it presents a contaminant in the end products. In order to analytically validate the products, species specific reagents are needed that react with bovine IgG exclusively. Our attempts to find such commercially available reagents failed. Here, we report the production of species specific mAbs which recognize bovine IgG even in the presence of excess amount of mouse IgG. We present five mAbs: Bsi4028, Bsi4032, Bsi4033, Bsi4034 and Bsi4035 suitable to determine the presence of bovine IgG contamination via ELISA or immunoblotting in bioreactor derived mouse mAb preparations. To quantitate bovine IgG content we developed sensitive sandwich ELISAs capable to detect bovine IgG contaminant in the ng/ml (~10-11M/l) range. Finally, we show that bovine IgG is efficiently removed from bioreactor produced mouse mAb preparation via affinity depletion columns prepared with Bsi4028, Bsi4032, Bsi4033, Bsi4034, Bsi4035 mAbs.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Bovine IgG contamination Detection Monoclonal antibody Purification
Megjelenés:	Journal of Immunological Methods. - 438 (2016), p. 26-34. -
További szerzők:	Lázár József Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Balogh Attila Ferenczi Annamária (1986-) (molekuláris biológus, mikrobiológus) Sós Endre Takács László (1955-) Kurucz István
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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