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001-es BibID:	BIBFORM095934
Első szerző:	Buglyó Gergely (genetikus)
Cím:	miRNA Profiling of Hungarian Regressive Wilms' Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR) / Gergely Buglyó, Zsófia Magyar, Éva Romicsné Görbe, Rita Bánusz, Monika Csóka, Tamás Micsik, Márta Mezei, Jaxi Ayman Shawky Yani, Péter Varga, Zoltán Sápi, Bálint Nagy
Dátum:	2021
ISSN:	1643-3750
Megjegyzések:	Background: Wilms' tumor is a common renal malignancy of early childhood with a generally favorable prognosis depending upon histological subtype. It is becoming increasingly clear that differences in miRNA (microRNA) expression signature represent important clues helping us predict a tumor's response to chemotherapy. In our study, we aimed to reveal miRNAs deregulated in regressive Wilms' tumors from FFPE (formalin-fixed, paraffin-embedded) samples, also showing whether such samples are reliable miRNA sources in Wilms' tumor. Material/Methods: Samples from 8 Hungarian patients (3 males, 5 females, aged 1 to 7 years) were analyzed by qRT-PCR (quantitative real-time PCR). A PCR array was used in a pilot experiment, and selected miRNAs (miR-128-3p, miR-184, miR-194-5p, miR-203a) were studied in the rest of the samples using individual primers. Results: miR-194-5p was underexpressed in all tumor samples. miR-184 and miR-203a were underexpressed in 7 cases, the exception being a case with a high ratio of necrotic blastemal tissue. Results obtained with miR-1283p are difficult to interpret due to varying directions of expression changes. Conclusions: We conclude that a downregulation of miR-184, miR-194-5p, and miR-203a expression is observed in both regressive and blastemal tumors, but larger-scale studies are needed to confirm whether the degree of their underexpression correlates with the number of blastemal elements in a sample. In most of our FFPE samples aged up to 9 years, RNA extraction provided miRNA with quantity and quality sufficient for qRT-PCR-based analysis, emphasizing the relevance of pathological archives as miRNA sources in future studies.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk MIRN184 microRNA Human MIRN194 microRNAHuman MIRN203 Human microRNA Real-Time Polymerase Chain Reaction Wilms Tumor
Megjelenés:	Medical Science Monitor. - 27 (2021), p. e932731-1-e932731-9. -
További szerzők:	Magyar Zsófia Görbe Éva Bánusz Rita Csóka Mónika Micsik Tamás Mezei Márta Yani, Jaxi Ayman Shawky Varga Péter (szülész-nőgyógyász) Sápi Zoltán Nagy Bálint (1956-) (molekuláris genetikus)
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