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001-es BibID:	BIBFORM111408
035-os BibID:	(scopus)85148518915 (wos)000936836300002
Első szerző:	Járvás Gábor (vegyészmérnök)
Cím:	Microbead-based extracorporeal immuno-affinity virus capture : a feasibility study to address the SARS-CoV-2 pandemic / Jarvas Gabor, Szerenyi Dora, Jankovics Hajnalka, Vonderviszt Ferenc, Tovari Jozsef, Takacs Laszlo, Foldes Fanni, Somogyi Balazs, Jakab Ferenc, Guttman Andras
Dátum:	2023
ISSN:	0026-3672
Megjegyzések:	In this paper, we report on the utilization of micro-technology based tools to fight viral infections. Inspired by various hemoperfusion and immune-affinity capture systems, a blood virus depletion device has been developed that offers highly efficient capture and removal of the targeted virus from the circulation, thus decreasing virus load. Single-domain antibodies against the Wuhan (VHH-72) virus strain produced by recombinant DNA technology were immobilized on the surface of glass micro-beads, which were then utilized as stationary phase. For feasibility testing, the virus suspension was flown through the prototype immune-affinity device that captured the viruses and the filtered media left the column. The feasibility test of the proposed technology was performed in a Biosafety Level 4 classified laboratory using the Wuhan SARS-CoV-2 strain. The laboratory scale device actually captured 120,000 virus particles from the culture media circulation proving the feasibility of the suggested technology. This performance has an estimated capture ability of 15 million virus particles by using the therapeutic size column design, representing three times over-engineering with the assumption of 5 million genomic virus copies in an average viremic patient. Our results suggested that this new therapeutic virus capture device could significantly lower virus load thus preventing the development of more severe COVID-19 cases and consequently reducing mortality rate.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Microchimica Acta. - 190 : 3 (2023), p. 1-9. -
További szerzők:	Szerényi Dóra Jankovics Hajnalka Vonderviszt Ferenc Tóvári József Takács László (1955-) (orvos) Földes Fanni Vivien (virológus) Somogyi Balázs Jakab Ferenc (1977-2024) (biológus) Guttman András (1954-) (vegyészmérnök)
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