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001-es BibID:	BIBFORM093483
035-os BibID:	(Scopus)85104748956 (WOS)000647236400001
Első szerző:	Rácz Hanna Viktória (biológus)
Cím:	How to characterize a strain? Clonal heterogeneity in industrial influences both phenotypes and heterogeneity in phenotypes / Hanna Viktória Rácz, Fezan Mukhtar, Alexandra Imre, Zoltán Rádai, Andreas Károly Gombert, Tamás Rátonyi, János Nagy, István Pócsi, Walter P. Pfliegler
Dátum:	2021
ISSN:	0749-503X
Megjegyzések:	Populations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is non?trivial to assess methodologically. The necessary practice of isolating single cell colonies and thus, subclone lineages for establishing, transferring, and using a strain results in single?cell bottlenecks with a generally neglected effect on the characteristics of the strain itself. Here, we present evidence that various subclone lineages for industrial yeasts sequenced for recent genomic studies show considerable differences, ranging from loss?of?heterozygosity to aneuploidies. Subsequently, we assessed whether phenotypic heterogeneity is also observable in industrial yeast, by individually testing subclone lineages obtained from products. Phenotyping of industrial yeast samples and their newly isolated subclones showed that single?cell bottlenecks during isolation can indeed considerably influence the observable phenotype. Next, we decoupled fitness distributions on the level of individual cells from clonal interference by plating single cell colonies and quantifying colony area distributions. We describe and apply an approach using statistical modeling to compare the heterogeneity in phenotypes accross samples and subclone lineages. One strain was further used to show how individual subclonal lineages are remarkably different not just in phenotype, but also in the level of heterogeneity in phenotype. With these observations, we call attention to the fact that choosing an initial clonal lineage from an industrial yeast strain may vastly influence downstream performances and observations on karyotype, phenotype, and also on heterogeneity.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk aneuploidy domestication Fermented foods and beverages genomic structural variation phenotypic variability Saccharomyces boulardii
Megjelenés:	Yeast. - 38 : 8 (2021), p. 453-470. -
További szerzők:	Mukhtar, Fezan Imre Alexandra (1992-) (biomérnök, biotechnológus) Rádai Zoltán (1991-) (biológus) Gombert Andreas Károly Rátonyi Tamás (1967-) (agrármérnök) Nagy János (1951-) (agrármérnök, mérnök-tanár) Pócsi István (1961-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:	GINOP-2.2.1-15-2016-00001 GINOP NKFIH-1150-6/2019 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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