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001-es BibID:	BIBFORM131775
Első szerző:	Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:	Propagation of [D1,2]-type spliceosomal twin introns (stwintrons) in Hypoxylaceae and Xylariaceae fungi / Erzsébet Fekete, Norbert Ág, Viktória Ág-Rácz, Alexandra Márton, Erzsébet Sándor, Claudio Scazzocchio, Michel Flipphi, Levente Karaffa
Dátum:	2025
ISSN:	2165-0497
Megjegyzések:	[D1,2] stwintrons consist of nested U2 introns, where an internal intron splits the 5·-donor of an external intron between its first and second nucleotide. Almost all stwintrons described to date are of the [D1,2] type, suggesting unique means for their duplication. Sequence-similar [D1,2] stwintrons are typically integrated at new intron positions, specific for one species. Two hundred eighty-eight sequence-similar [D1,2] stwintrons were identified in the genomes of 14 Xylariales. Occasional missplicing was apparent, where almost the entire stwintron was excised as one canonical intron. Near-identical sister stwintrons were identified in Xylaria sp. MSU SB201401 and Xylaria longipes that share near-terminal inverted repeat elements of 10-nt length named 5·-NTIRE-10 and 3·-NTIRE-10, which are complementary as RNA. Complementary NTIRE-10 partners were also present in three Hypoxylaceae species. These NTIRE-10s can form a near-terminal double-stranded RNA stem structure that brings in close proximity the terminal G's of the [D1,2] stwintron and of its alternative misspliced intron. The exact folding of the interior stwintron RNA appears irrelevant. Ten of the stwintrons with complementary NTIRE-10s are present in Xylaria sp. MSU SB201401, implying that [D1,2] stwintron duplication occurs frequently in this species.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk spliceosomal introns splicing [D1,2] stwintron propagation Xylariales near-identical stwintrons near-terminal double-stranded RNA stem structure D1,2] missplicing using distal splice sites
Megjelenés:	Microbiology Spectrum. - [Epub ahead of print] (2025), p. 1-16. -
További szerzők:	Ág Norbert (1987-) (okleveles biomérnök) Ág-Rácz Viktória (1991-) (mikrobiológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Scazzocchio, Claudio Flipphi, Michel (1964-) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Pályázati támogatás:	K 146406 NKFIH K 138489 NKFIH
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM101910
035-os BibID:	(WoS)000787051700001 (Scopus)85129122957
Első szerző:	Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:	Unique and Repeated Stwintrons (Spliceosomal Twin Introns) in the Hypoxylaceae / Erzsébet Fekete, Fruzsina Pénzes, Norbert Ág, Viktória Ág-Rácz, Erzsébet Sándor, Claudio Scazzocchio, Michel Flipphi, Levente Karaffa
Dátum:	2022
ISSN:	2309-608X
Megjegyzések:	Introns are usually non-coding sequences interrupting open reading frames in pre-mRNAs [D1,2]. Stwintrons are nested spliceosomal introns, where an internal intron splits a second donor sequence into two consecutive splicing reactions leading to mature mRNA. In Hypoxylon sp. CO27-5, 36 highly sequence-similar [D1,2] stwintrons are extant (sister stwintrons). An additional 81 [D1,2] sequence-unrelated stwintrons are described here. Most of them are located at conserved gene positions rooted deep in the Hypoxylaceae. Absence of exonic sequence bias at the exon?stwintron junctions and a very similar phase distribution were noted for both groups. The presence of an underlying sequence symmetry in all 117 stwintrons was striking. This symmetry, more pronounced near the termini of most of the full-length sister stwintrons, may lead to a secondary structure that brings into close proximity the most distal splice sites, the donor of the internal and the acceptor of the external intron. The Hypoxylon stwintrons were overwhelmingly excised by consecutive splicing reactions precisely removing the whole intervening sequence, whereas one excision involving the distal splice sites led to a frameshift. Alternative (mis)splicing took place for both sister and uniquely occurring stwintrons. The extraordinary symmetry of the sister stwintrons thus seems dispensable for the infrequent, direct utilisation of the distal splice sites.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk spliceosomal introns splicing; stwintrons comparative genomics stwintron dissemination stwintron propagation internal symmetry single-stranded RNA secondary structure prediction Hypoxylon sp. CO27-5 Hypoxylaceae
Megjelenés:	Journal of Fungi. - 8 : 4 (2022), p. 1-23. -
További szerzők:	Pénzes Fruzsina (1995-) (okleveles biomérnök) Ág Norbert (1987-) (okleveles biomérnök) Ág-Rácz Viktória (1991-) (mikrobiológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Scazzocchio, Claudio Flipphi, Michel (1964-) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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