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001-es BibID:	BIBFORM025200
Első szerző:	Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:	Regulation of formation of the intracellular β-galactosidase activity of Aspergillus nidulans / Erzsébet Fekete, Levente Karaffa, Erzsébet Sándor, Bernhard Seiboth, Sándor Biró, Attila Szentirmai, Christian P. Kubicek
Dátum:	2002
Megjegyzések:	The regulation of formation of the single intracellular beta-galactosidase activity of Aspergillus nidulans was investigated. beta-Galactosidase was not formed during growth on glucose or glycerol, but was rapidly induced during growth on lactose or D-galactose. L-Arabinose, and - with lower efficacy - D-xylose also induced beta-galactosidase activity. Addition of glucose to cultures growing on lactose led to a rapid decrease in beta-galactosidase activity. In contrast, in cultures growing on beta-galactose, addition of glucose decreased the activity of beta-galactosidase only slightly. Glucose inhibited the uptake of lactose, but not of D-galactose, and required the carbon catabolite repressor CreA for this. In addition, CreA also repressed the formation of basal levels of beta-galactosidase and partially interfered with the induction of beta-galactosidase by D-galaclose, L-arabinose, and D-xylose. D-Galactose phosphorylation was not necessary for beta-galactosidase induction, since induction by D-galactose occurred in an A. nidulans mutant defective in galactose kinase, and by the non-metabolizable D-galactose analogue fucose in the wild-type strain. Interestingly, a mutant in galactose-1-phosphate uridylyl transferase produced beta-galactosidase at a low, constitutive level even on glucose and glycerol and was no longer inducible by D-galactose, whereas it was still inducible by L-arabinose. We conclude that biosynthesis of the intracellular beta-galactosidase of A. nidulans is regulated by CreA,. partially repressed by galactose-1-phosphate uridylyl transferase, and induced by D-galactose and L-arabinose in independent ways.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Archives of Microbiology. - 179 : 1 (2002), p. 7-14. -
További szerzők:	Karaffa Levente (1971-) (biotechnológus, egyetemi tanár) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Seiboth, Bernhard Biró Sándor (1949-) (molekuláris genetikus) Szentirmai Attila (1930-) (mikrobiológus) Kubicek, Christian P.
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL
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