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001-es BibID:	BIBFORM132834
035-os BibID:	(WoS)001593114200001
Első szerző:	Diószegi Róbert (vegyész)
Cím:	Highly active superoxide dismutase mimic: pyridine carboxamide-based copper(II) complexes / Róbert Diószegi, Andrea Guidetti, Norbert Ág, Ilenia Serra, Dóra Szalóki, Dóra Bonczidai-Kelemen, Nóra V. May, Erzsébet Fekete, Levente Karaffa, István Fábián, Sabine Van Doorslaer, Norbert Lihi
Dátum:	2025
ISSN:	2052-1553
Megjegyzések:	Pyridine-based ligands functionalized with one (PyHis) or two histidine (PydiHis) moieties were synthesized, and their copper(II) complexes were studied as novel antioxidant systems. The complexes were characterized by pH-potentiometric, spectroscopic (UV-vis, circular dichroism, continuous-wave and pulsed electron paramagnetic spectroscopy (EPR)), computational (DFT) and X-ray diffraction methods. The complexes show high thermodynamic stability. The PyHis complex possesses a (COO?,Npy,N?,NIm) donor set, while PydiHis binds copper(II) via a (NIm,Npy,N?,NIm) donor set at physiological pH. The PyHis and PydiHis complexes of copper(II) exhibit negligible (IC50 = 147 ? 15 ?M) and outstanding (IC50 = 0.64 ? 0.07 ?M) superoxide dismutase (SOD) activity, respectively. The noted difference is attributed to the structural flexibility of the latter complex as confirmed by pulsed EPR spectroscopy. The antioxidant activity of the copper(II) complexes was also tested in vivo by monitoring their effects on the mycelial growth of Aspergillus niger under oxidative stress. Both complexes were able to protect the cells under these conditions; however, the copper(II) complex of PydiHis was more effective in accordance with its excellent SOD activity. Consequently, the copper(II) complex of PydiHis stands out as an efficient, low-molecular-weight SOD mimic combining high catalytic activity with proven biological efficacy under oxidative stress conditions, making it a promising candidate for medical applications.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Inorganic Chemistry Frontiers. - "Accepted by Publisher" (2025), p. 1-16. -
További szerzők:	Guidetti, Andrea Ág Norbert (1987-) (okleveles biomérnök) Serra, Ilenia Vargáné Szalóki Dóra (1989-) (vegyész) Bonczidai-Kelemen Dóra (1995-) May Nóra Veronika Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár) Fábián István (1956-) (vegyész) Van Doorslaer, Sabine Lihi Norbert (1990-) (vegyész)
Pályázati támogatás:	K-139140 OTKA K-146406 OTKA K-138489 OTKA 2021-4.1.2-NEMZ_KI-2024-00042 Egyéb DETKA Egyéb EKÖP-24-3 Egyéb
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001-es BibID:	BIBFORM103023
035-os BibID:	(WoS)000843102800001 (Scopus)85136210735
Első szerző:	Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:	Bioreactor as the root cause of the "manganese effect" during Aspergillus niger citric acid fermentations / Fekete Erzsébet, Bíró Vivien, Márton Alexandra, Bakondi-Kovács István, Németh Zoltán Sándor Erzsébet, Kovács Béla, Fábián István, Kubicek Christian P., Tsang Adrian, Karaffa Levente
Dátum:	2022
ISSN:	2296-4185
Megjegyzések:	High-yield citric acid production by the filamentous Ascomycete fungus Aspergillus niger requires a combination of extreme nutritional conditions, of which maintaining a low manganese (II) ion concentration (<5 ?g L?1 ) is a key feature. Technical-scale production of citric acid predominantly uses stainless- steel tank fermenters, but glass bioreactors used for strain improvement and manufacturing process development also contain stainless steel components, in which manganese is an essential alloying element. We show here that during citric acid fermentations manganese (II) ions were leaching from the bioreactor into the growth media, resulting in altered fungal physiology and morphology, and significant reduction of citric acid yields. The leaching of manganese (II) ions was dependent on the fermentation time, the acidity of the culture broth and the sterilization protocol applied. Manganese (II) ion leaching was partially mitigated by electrochemical polishing of stainless steel components of the bioreactor. High concentrations of manganese (II) ions during early cultivation led to a reduction in citric acid yield. However, the effect of manganese (II) ions on the reduction of citric acid yield diminished towards the second half of the fermentation. Since maintaining low concentrations of manganese (II) ions is costly, the results of this study can potentially be used to modify protocols to reduce the cost of citric acid production.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Aspergillus niger manganese ions citric acid stainless steel metal ions leaching fungal morphology
Megjelenés:	Frontiers in Bioengineering and Biotechnology. - 10 (2022), p. 1-12. -
További szerzők:	Bíró Vivien (1994-)c(mikrobiológus) Márton Alexandra (1994-) (mikrobiológus) Bakondi-Kovács István (1963-) (mikrobiológus) Németh Zoltán (1987-) (biomérnök) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Kovács Béla (1963-) (mezőgazdasági kémia, angol szakfordító) Fábián István (1956-) (vegyész) Kubicek, Christian P. Tsang, Adrian Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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