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001-es BibID:BIBFORM081935
035-os BibID:(WOS)000499182500001 (Scopus)85075277364
Első szerző:Szabó Krisztina (molekuláris biológus)
Cím:Deletion of the fungus specific protein phosphatase Z1 exaggerates the oxidative stress response in Candida albicans / Krisztina Szabó, Ágnes Jakab, Szilárd Póliska, Katalin Petrényi, Katalin Kovács, Lama Hasan Bou Issa, Tamás Emri, István Pócsi, Viktor Dombrádi
Megjegyzések:Background:Candida albicansis an opportunistic pathogen which is responsible for widespread nosocomialinfections. It encompasses a fungus specific serine/threonine protein phosphatase gene, CaPPZ1 that is involvedin cation transport, cell wall integrity, oxidative stress response, morphological transition, and virulence accordingto the phenotypes of thecappz1deletion mutant.Results:We demonstrated that a short-term treatment with a sublethal concentration oftert-butyl hydroperoxidesuppressed the growth of the fungal cells without affecting their viability, both in thecappz1mutant and in thegenetically matching QMY23 control strains. To reveal the gene expression changes behind the above observationswe carried out a global transcriptome analysis. We used a pilot DNA microarray hybridization together withextensive RNA sequencing, and confirmed our results by quantitative RT-PCR. Novel functions of the CaPpz1enzyme and oxidative stress mechanisms have been unraveled. The numbers of genes affected as well as theamplitudes of the transcript level changes indicated that the deletion of the phosphatase sensitized the responseofC. albicansto oxidative stress conditions in important physiological functions like membrane transport, cellsurface interactions, oxidation-reduction processes, translation and RNA metabolism.Conclusions:We conclude that in the wild typeC. albicansCaPPZ1 has a protective role against oxidative damage.We suggest that the specific inhibition of this phosphatase combined with mild oxidative treatment could be afeasible approach to topical antifungal therapy.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Candida albicans
Protein phosphatase Z1
Deletion mutant
Oxidative stress
tert-butyl hydoperoxide
DNA microarray
Quantitative RT-PCR
Megjelenés:Bmc Genomics. - 20 (2019), p. 1-17. -
További szerzők:Jakab Ágnes (1987-) (biológus) Póliska Szilárd (1978-) (biológus) Petrényi Katalin (1988-) (Ph.D hallgató) Kovács Katalin (1978-) (biokémikus) Issa, Lama Hasan Bou Emri Tamás (1969-) (biológus) Pócsi István (1961-) (vegyész) Dombrádi Viktor (1953-) (biokémikus)
Pályázati támogatás:NKFIH-K108989
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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