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001-es BibID:BIBFORM065024
Első szerző:Kovács Csilla (élelmiszerbiztonsági és minőségi mérnök)
Cím:Grapevine trunk diseases in the Tokaj wine region, Hungary / Kovács C., Szojka A., Bihari Z., Sándor E., Peles F.
Megjegyzések:Grapevine trunk diseases (GTD) are one of the most important diseases in vineyards worldwide, which can be found in Hungarian vineyards as well. GTD are caused by a variety of pathogen fungi, like: Phaeoacremonium aleophilum, Phaeomoniella chlamydospora, Eutypa lata, Fomitiporia mediterranae, Diplodia seriata. There is very limited information about the occurrence of different pathogens causing GTD in the Tokaj wine region nowadays. Currently there is no effective fungicide, which could be used against the disease.The objective of the research were estimated the occurrence of grapevine trunk diseases in Tokaj wine region in cooperation with local specialists; furthermore, to isolate and identify fungi associated with symptoms of GTD in vineyards.Tokaj wine region have indigenous grape varieties (Hárslevelű and Furmint). The samples came from the Bakonyi area (101 samples). We cooperated with the staffs of the Research Institute for Viticulture and Oenology (Tokaj) in the collection of the grapevine trunk samples. Pure fungal cultures were used for morphological (colony formation, pigment production and the shape of the spore) and molecular identification of fungal species. The woody tissue samples were cut in a small part, and were disinfected with 10% Neomagnol (tosylchloramide sodium B) solution for 10 minutes, than were washed twice with sterile distilled water. Afterwards they were put on malt extract agar medium and incubated at room temperature (25 ?C) for 3-8 days. The isolates were maintained on potato-dextrose agar (PDA) at 4 ?C. Fungal DNA was extracted from three days old fungal mycelia growth on 50 ml potato dextrose broth on a rotary shaker (125 rpm) at room temperature, and was harvested by vacuum filtration. MagNaLyser (Roche) was used for disruption, and isolation was carried with NucleoSpin Plant II (Macherey-Nagel). The ITS4 and ITS5 primers were used for PCR amplification of the internal transcribed spacer regions. Purified amplicons were sequenced by MWG Biotech Company in Germany. The sequences were aligned with deponated reference sequences with Clustal X program, and manually corrected with GeneDoc. Phylogenetic analyses were performed with MEGA 5.05 (Kovács et al., 2014).The foliar symptoms could be observed on 0.46% (Szemere-dűlő), 3.49% (Dorgó-dűlő); 4.27% (Szarvas-dűlő) and 0.34% (Várhegy) of the examined trunks. However, dieback could be detected only in few cases. More than one hundred fungi were isolated in case of Bakonyi area from symptomatic woody tissues. In case of Bakonyi area the majority of species (72.3%) were determined as member of Botryosphaeriaceae, sixty-six samples were determined seventy-three samples as Diplodia seriata, and six samples as Botryosphaeria stevensii. Other fungi, like Alternaria (four), Mucor ramossimus (nine), Phoma sp. (one), Diaporthe (two) and Xylaria (seven),Gibberella zea (one),Pheniophora sp.(one), Xanthomendoza sp. (one) were also identified from grapevine trunk samples.
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok idézhető absztrakt
Grapevine Trunk Diseases
Tokaj wine region
Megjelenés:Integrative Food, Nutrition and Metabolism 1 : 1 (2014), p. 60. -
További szerzők:Bérczesné Szojka Anikó (1987-) (biomérnök) Bihari Zoltán (1967-) (biológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Pályázati támogatás:TÁMOP-4.2.4.A/2-11-1-2012-0001
Sándor Erzsébet munkáját a Debreceni Egyetem belső kutatási pályázata támogatta
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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