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1.
001-es BibID:
BIBFORM107554
035-os BibID:
(scopus)84874195115 (wos)000209501300326
Első szerző:
Fuertes Marraco, Silvia A.
Cím:
Novel Murine Dendritic Cell Lines : a Powerful Auxiliary Tool for Dendritic Cell Research / Fuertes Marraco Silvia A., Grosjean Frédéric, Duval Anaïs, Rosa Muriel, Lavanchy Christine, Ashok Devika, Haller Sergio, Otten Luc A., Steiner Quynh-Giao, Descombes Patrick, Luber Christian A., Meissner Felix, Mann Matthias, Szeles Lajos, Reith Walter, Acha-Orbea Hans
Dátum:
2012
ISSN:
1664-3224
Megjegyzések:
Research in vitro facilitates discovery, screening, and pilot experiments, often preceding research in vivo. Several technical difficulties render Dendritic Cell (DC) research particularly challenging, including the low frequency of DC in vivo, thorough isolation requirements, and the vulnerability of DC ex vivo. Critically, there is not as yet a widely accepted human or murine DC line and in vitro systems of DC research are limited. In this study, we report the generation of new murine DC lines, named MutuDC, originating from cultures of splenic CD8? conventional DC (cDC) tumors. By direct comparison to normal WT splenic cDC subsets, we describe the phenotypic and functional features of the MutuDC lines and show that they have retained all the major features of their natural counterpart in vivo, the splenic CD8? cDC. These features include expression of surface markers Clec9A, DEC205, and CD24, positive response to TLR3 and TLR9 but not TLR7 stimuli, secretion of cytokines, and chemokines upon activation, as well as cross-presentation capacity. In addition to the close resemblance to normal splenic CD8? cDC, a major advantage is the ease of derivation and maintenance of the MutuDC lines, using standard culture medium and conditions, importantly without adding supplementary growth factors or maturation-inducing stimuli to the medium. Furthermore, genetically modified MutuDC lines have been successfully obtained either by lentiviral transduction or by culture of DC tumors originating from genetically modified mice. In view of the current lack of stable and functional DC lines, these novel murine DC lines have the potential to serve as an important auxiliary tool for DC research.
Tárgyszavak:
Természettudományok
Biológiai tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
dendritic cell
antigen-presenting cells
Megjelenés:
Frontiers in Immunology. - 3 (2012), p. 1-25. -
További szerzők:
Grosjean, Frédéric
Duval, Anaïs
Rosa, Muriel
Lavanchy, Christine
Ashok, Devika
Haller, Sergio
Otten, Luc A.
Steiner, Quynh-Giao
Descombes, Patrick
Luber, Christian A.
Meissner, Felix
Mann, Matthias
Széles Lajos (1971-) (molekuláris biológus)
Reith, Walter
Acha-Orbea, Hans
Internet cím:
Szerző által megadott URL
DOI
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Saját polcon:
2.
001-es BibID:
BIBFORM107547
035-os BibID:
(scopus)84937718267 (wos)000358070400031
Első szerző:
Széles Lajos (molekuláris biológus)
Cím:
TLR3-Mediated CD8+ Dendritic Cell Activation Is Coupled with Establishment of a Cell-Intrinsic Antiviral State / Széles Lajos, Meissner Felix, Dunand-Sauthier Isabelle, Thelemann Christoph, Hersch Micha, Singovski Simon, Haller Sergio, Gobet Florian, Fuertes Marraco Silvia A., Mann Matthias, Garcin Dominique, Acha-Orbea Hans, Reith Walter
Dátum:
2015
ISSN:
0022-1767 1550-6606
Megjegyzések:
Because of their unique capacity to cross-present Ags to CD8+ T cells, mouse lymphoid tissue?resident CD8+ dendritic cells (DCs) and their migratory counterparts are critical for priming antiviral T cell responses. High expression of the dsRNA sensor TLR3 is a distinctive feature of these cross-presenting DC subsets. TLR3 engagement in CD8+ DCs promotes cross-presentation and the acquisition of effector functions required for driving antiviral T cell responses. In this study, we performed a comprehensive analysis of the TLR3-induced antiviral program and cell-autonomous immunity in CD8+ DC lines and primary CD8+ DCs. We found that TLR3-ligand polyinosinic-polycytidylic acid and human rhinovirus infection induced a potent antiviral protection against Sendai and vesicular stomatitis virus in a TLR3 and type I IFN receptor?dependent manner. Polyinosinic-polycytidylic acid?induced antiviral genes were identified by mass spectrometry?based proteomics and transcriptomics in the CD8+ DC line. Nanostring nCounter experiments confirmed that these antiviral genes were induced by TLR3 engagement in primary CD8+ DCs, and indicated that many are secondary TLR3-response genes requiring autocrine IFN-? stimulation. TLR3-activation thus establishes a type I IFN?dependent antiviral program in a DC subtype playing crucial roles in priming adaptive antiviral immune responses. This mechanism is likely to shield the priming of antiviral responses against inhibition or abrogation by the viral infection. It could be particularly relevant for viruses detected mainly by TLR3, which may not trigger type I IFN production by DCs that lack TLR3, such as plasmacytoid DCs or CD8? DCs.
Tárgyszavak:
Természettudományok
Biológiai tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
antivirális immunitás
interferon
TLR3
Megjelenés:
Journal Of Immunology. - 195 : 3 (2015), p. 1025-1033. -
További szerzők:
Meissner, Felix
Dunand-Sauthier, Isabelle
Thelemann, Christoph
Hersch, Micha
Singovski, Simon
Haller, Sergio
Gobet, Florian
Fuertes Marraco, Silvia A.
Mann, Matthias
Garcin, Dominique
Acha-Orbea, Hans
Reith, Walter
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
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