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001-es BibID:BIBFORM103569
035-os BibID:(WoS)000851426500001 (Scopus)85146322280
Első szerző:Rebenku István
Cím:Taking molecular pathology to the next level : whole slide multicolor confocal imaging with the Pannoramic Confocal digital pathology scanner / István Rebenku, Ferenc A. Bartha, Tamás Katona, Barbara Zsebik, Géza Antalffy, Lili Takács, Béla Molnár, György Vereb
ISSN:1552-4922 1552-4930
Megjegyzések:Abstract The emergence and fast advance of digital pathology allows the acquisition, digital storage, interactive recall and analysis of morphology at the tissue level. When applying immunohistochemistry, it also affords the correlation of morphology with the expression of one or two specific molecule of interest. The rise of fluorescence pathology scanners expands the number of detected molecules based on multiplex labelling. The Pannoramic Confocal (created by 3DHistech, Hungary) is a first-of-the-kind digital pathology scanner that affords not only multiplexed fluorescent detection on top of conventional transmission imaging, but also confocality. We have benchmarked this scanner in terms of stability, precision, light efficiency, linearity and sensitivity. X-Y stability and relocalisation precision were well below resolution limit (?50 nm). Light throughput in confocal mode was 4-5 times higher than that of a point scanning confocal microscope, yielding similar calculated confocal intensities but with the potential for improving signal to noise ratio or scan speed. Response was linear with R2 ?0.9996. Calibrated measurements showed that using indirect labeling ? 2000 molecules per cell could be well detected and imaged on the cell surface. Both standard-based and statistical post-acquisition flatfield corrections are implemented. We have also measured the point spread function (PSF) of the instrument. The dimensions of the PSF are somewhat larger and less symmetric than of the theoretical PSF of a conventional CLSM, however, the spatial homogeneity of these parameters allows for obtaining a specific system PSF for each optical path and using it for optional on-the-fly deconvolution. In conclusion, the Pannoramic Confocal provides sensitive, quantitative widefield and confocal detection of multiplexed fluorescence signals, with optical sectioning and 3D reconstruction, in addition to brightfield transmission imaging. High speed scanning of large samples, analysis of tissue heterogeneity, and detection of rare events open up new ways for quantitatively analyzing tissue sections, organoid cultures or large numbers of adherent cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
3-dimensional virtual slide
digital pathology
multispectral fluorescence imaging
widefield aperture correlated confocal imaging
Megjelenés:Cytometry Part A. - 103 : 3 (2023), p. 198-207. -
További szerzők:Bartha Ferenc Katona Tamás (1991-) (informatikus) Zsebik Barbara (1977-) (biofizikus) Antalffy Géza Takács Lili (1969-) (szemész) Molnár Béla Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:GINOP-2.2.1-15-2017-00072
OTKA K135938
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001-es BibID:BIBFORM102343
Első szerző:Zsebik Barbara (biofizikus)
Cím:ErbB2 homodimerization and activation in Herceptin resistant and sensitive cell lines / Zsebik B., Petrás M., Isola J., Szöllősi J., Vereb G.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Cytometry. - 59A : 1 (2004), p. 113. -
További szerzők:Petrás Miklós (1977-) (orvos) Isola, Jorma Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (absztraktok, könyvfejezetek)
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