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001-es BibID:BIBFORM002772
Első szerző:Zsebik Barbara (biofizikus)
Cím:Photodynamic therapy combined with a cysteine proteinase inhibitor synergistically decrease VEGF production and promote tumour necrosis in a rat mammary carcinoma / Zsebik B., Symonowicz K., Saleh Y., Ziolkowski P., Bronowicz A., Vereb G.
Dátum:2007
Megjegyzések:Photodynamic therapy (PDT) and inhibition of cathepsin B proteases by cystatin (cysteine proteinase inhibitor, CPI) are potential new tumour treatment modalities. We have investigated the efficacy of PDT and CPI alone and in combination on a solid mammary carcinoma transplanted into Wistar rats. MATERIALS AND METHODS: Intraperitoneally injected single doses of chlorine e6 or HpD as photosensitizers were excited at 630 nm (90 J/cm(2)). CPI (500 micro g per animal) was injected around the tumour daily during the 8-day treatment. Inoculation of tumour was either on day 1 of the protocol, or 8 days before. On day 8, tumour size was measured, tumour necrosis and vascularization were determined based on haematoxylin and eosin (H&E)-stained sections and serum vascular endothelial growth factor (VEGF) levels measured using an enzyme-linked immunosorbent assay kit. RESULTS: No differences (two-way anova) were found for treatments started with various time lags. At doses where CPI or PDT alone had no or negligible effect, their combination caused a marked (P < 0.001) decrease in serum VEGF, paralleled by a significant decrease in tumour size and number of capillary vessels, and a significant increase in necrosis (up to 80% of the tumour tissue). CONCLUSIONS: The combination of PDT and CPI could be a useful approach in tumour therapy as the two agents appear to be synergistic and probably decrease VEGF production by the tumour tissue
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Animals
Biophysics
biosynthesis
blood supply
Capillaries
Carcinoma
Comparative Study
Cystatins
Cysteine
Cysteine Proteinase Inhibitors
Dose-Response Relationship,Drug
drug therapy
Enzyme-Linked Immunosorbent Assay
Female
Hungary
Mammary Neoplasms,Experimental
methods
Necrosis
Neoplasm Transplantation
pathology
pharmacology
Photochemotherapy
Rats
Rats,Wistar
Research
Support
therapeutic use
therapy
Vascular Endothelial Growth Factor A
Megjelenés:Cell Proliferation. - 40 : 1 (2007), p. 38-49. -
További szerzők:Symonowicz, K. Saleh, Y. Ziolkowski, P. Bronowicz, A. Vereb György (1965-) (biofizikus, orvos)
Internet cím:DOI
elektronikus változat
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