Találati lista | Tudóstér

001-es BibID:	BIBFORM055871
Első szerző:	Bácsi Attila (immunológus)
Cím:	Antigen-independent mitochondria-driven release of biogenic amines from RBL-2H3 cells / Bácsi Attila, Chodaczek Grzegorz, Dharajiya Nilesh, Sur Sanjiv, Hazra Tapas K., Boldogh István
Dátum:	2007
Tárgyszavak:	Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:	Journal of Allergy and Clinical Immunology. - 123 (2007), p. S118. -
További szerzők:	Chodaczek, Grzegorz Dharajiya, Nilesh G. Sur, Sanjiv Hazra, Tapas K. Boldogh István
Borító:
Saját polcon:

001-es BibID:	BIBFORM002003
Első szerző:	Bácsi Attila (immunológus)
Cím:	Increased ROS generation in subsets of OGG1 knockout fibroblast cells / Attila Bacsi, Grzegorz Chodaczek, Tapas K. Hazra, David Konkel, Istvan Boldogh
Dátum:	2007
Megjegyzések:	Oxoguanine DNA glycosylase (OGG1) is a major base excision repair protein responsible for excision of the mutagenic 8-oxoguanosine (8-oxoG) lesions from the genome. Despite OGG1's importance, the moderate phenotype of Ogg1-null (Ogg1_/_) mice is not well understood. This study addresses a mechanism by which Ogg1_/_ cells limit accumulation of 8-oxoG in their genome. Our data reveal that a subset of Ogg1_/_ cells shows higher ROS levels (HROS cells), while _85% of Ogg1_/_ cells exhibit physiological levels of ROS (LROS cells). Ogg1_/_ cells were sorted based on their DCF fluorescence intensity to obtain LROS and HROS cell cultures. LROS cultures proliferated at a rate comparable to Ogg1+/+ and gradually accumulated cells exhibiting increased ROS and 8-oxoG levels. LROS cells show a 2.8-fold increase in 8-oxoG level vs. HROS cells (7? 27-fold). Mitochondria of HROS cells released more H2O2 than LROS and Ogg1+/+ cells and were eliminated by apoptotic-like processes. These findings suggest that in the absence of OGG1, a surveillance system is activated that removes cells with extreme 8-oxoG levels from Ogg1_/_ cultures. Whether similar mechanisms exists in tissues of Ogg1_/_ mice is the focus of future investigations.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Ogg1 null fibroblast ROS 8-oxoguanine Mitochondria
Megjelenés:	Mechanisms of ageing and development. - 128 : 11-12 (2007), p. 637-649. -
További szerzők:	Chodaczek, Grzegorz Hazra, Tapas K. Konkel, David Boldogh István
Internet cím:	elektronikus változat DOI elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM010266
Első szerző:	Chodaczek, Grzegorz
Cím:	Ragweed pollen-mediated IgE-independent release of biogenic amines from mast cells via induction of mitochondrial dysfunction / Grzegorz Chodaczek, Attila Bácsi, Nilesh G. Dharajiya, Sanjiv Sur, Tapas K. Hazra, István Boldogh
Dátum:	2009
Megjegyzések:	Normal functions of mitochondria are required for physiological dynamics of cells, while their dysfunction contributes to development of various disorders including those of immune system. Here we demonstrate that exposure of mast cells to ragweed pollen extract increases production of H2O2 via mitochondrial respiratory complex III. These mitochondrial ROS (mtROS) enhance secretion of histamine and serotonin from mast cells, but not enzymes such as ?-hexosaminidase, independently from FcRI-generated stimuli. The release of biogenic amines is associated with inhibition of secretory granules' H+-ATPase activity, activation of PKC-? and microtubule-dependent motility, and it is independent from intracellular free Ca2+ levels. To asses differences from IgE-mediated mast cell degranulation we show that mtROS decrease antigen-triggered ?-hexosaminidase release, while they are synergistic with antigen-induced IL-4 production in sensitized cells. Taken together, these data indicate that mitochondrial dysfunction can act independently from adaptive immunity, as well as augments Th2-type responses. Pharmacological maintenance of physiological mitochondrial function could have clinical benefits in prevention and treatment of allergic diseases.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Mast cells Mitochondria Degranulation Reactive oxygen species
Megjelenés:	Molecular Immunology. - 46 : 13 (2009), p. 2505-2514. -
További szerzők:	Bácsi Attila (1967-) (immunológus) Dharajiya, Nilesh G. Sur, Sanjiv Hazra, Tapas K. Boldogh István
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM002001
Első szerző:	Chodaczek, Grzegorz
Cím:	Iron-mediated dismutation of superoxide anion augments antigen-induced allergic inflammation : effect of lactoferrin / Grzegorz Chodaczek, Alfredo Saavedra-Molina, Attila Bacsi, Marian L. Kruzel, Sanjiv Sur, Istvan Boldogh
Dátum:	2007
Megjegyzések:	The authors previously showed that pollen grain-, pollen grain extract-. and subpollen particle-induced allergic inflammation in lungs and eyes is robustly augmented by their intrinsic NAD(P)H oxidase activity. Here they sought to determine whether lactoferrin (LF), an iron-binding protein and immune modulator, decreases allergic inflammation induced by ragweed (Ambrosia artemisiifolia) pollen grain extract (RWE). MATERIAL/METHODS: The impact of LF on NAD(P)H oxidase in pollen grains and reactive oxygen species (ROS) levels in vitro and in the lungs of experimental animals was assessed by use of redox-sensitive probes and specific inhibitors. The influence of LF on RWE-induced allergic inflammation was determined in a mouse experimental model of asthma. RESULTS: The data show that the intrinsic NAD(P)H oxidase of pollen grains generates superoxide anion (O2-) and that LF does not alter its enzymatic activity, as shown by nitroblue tetrazolium and cytochrome c assays. On the other hand, LF significantly decreased H(2)- O(2)- and lipid peroxide (4-hydroxynoneal and malondialdehyde) levels in airway lining fluids and lung epithelium after intranasal challenge of naive or sensitized mice with RWE. Furthermore, a single dose of LF prevented/decreased the abundance of the RWE-induced robust accumulation of inflammatory and mucin-producing cells in airways and subepithelial compartments and decreased airway hyperreactivity. CONCLUSION: These data suggest that the reduced conversion of NAD(P)H oxidase-generated O(2)- into H(2)- O(2)- and/or OH, which in turn synergistically enhanced pollen antigen-induced airway inflammation, is due to the iron-binding capacity of LF. These results support the utility of LF in human allergic inflammatory disorders.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Lactoferrin allergy airway infl ammation oxidative stress
Megjelenés:	Postepy higieny i medycyny doswiadczalnej (Online). - 61 (2007), p. 268-278. -
További szerzők:	Saavedra-Molina, Alfredo Bácsi Attila (1967-) (immunológus) Kruzel, Marian L. Sur, Sanjiv Boldogh István
Internet cím:	elektronikus változat elektronikus változat
Borító:
Saját polcon: