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001-es BibID:	BIBFORM046595
035-os BibID:	PMID:11328823
Első szerző:	Briggs, Scott D.
Cím:	HIV-1 Nef promotes survival of myeloid cells by a Stat3-dependent pathway / Scott D. Briggs, Beata Scholtz, Jean-Marc Jacque, Simon Swingler, Mario Stevenson, Thomas E. Smithgall
Dátum:	2001
ISSN:	0021-9258 1083-351X
Megjegyzések:	Eppley Institute for Research in Cancer Nebraska Medical Center (Omaha, NE) Human immunodeficiency virus Nef is a small myristylated protein that plays a critical role in AIDS progression. Nef binds with high affinity to the SH3 domain of the myeloid-restricted tyrosine kinase Hck in vitro, identifying this Src-related kinase as a possible cellular target for Nef in macrophages. Here we show that Nef activates endogenous Hck in the granulocyte-macrophage colony-stimulating factor-dependent myeloid cell line, TF-1. Unexpectedly, Nef induced cytokine-independent TF-1 cell outgrowth and constitutive activation of the Stat3 transcription factor. Induction of survival required the Nef SH3 binding and membrane-targeting motifs and was blocked by dominant-negative Stat3 mutants. Nef also stimulated Stat3 activation in primary human macrophages, providing evidence for Stat3 as a Nef effector in a target cell for human immunodeficiency virus.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Biological Chemistry. - 276 : 27 (2001), p. 25605-25611. -
További szerzők:	Scholtz Beáta (1967-) (biokémikus, molekuláris biológus) Jacque, Jean-Marc Swingler, Simon Stevenson, Mario Smithgall, Thomas E.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM046593
035-os BibID:	PMID:8943301
Első szerző:	Scholtz Beáta (biokémikus, molekuláris biológus)
Cím:	Transcription of the transforming growth factor-beta2 gene is dependent on an E-box located between an essential cAMP response element/activating transcription factor motif and the TATA box of the gene / Beáta Scholtz, Michelle Kingsley-Kallesen, Angie Rizzino
Dátum:	1996
ISSN:	0021-9258 1083-351X
Megjegyzések:	Eppley Institute for Research in Cancer and Allied Diseases, Department of Pathology and Microbiology, University of Nebraska Medical Center (Omaha, Nebraska) Transforming growth factor-beta2 (TGF-beta2) is an important regulator of cell proliferation and differentiation; however, its transcriptional regulation is not well understood. Here we report characterization of an essential E-box motif, positioned at -50/-45 between a previously described functional cAMP response element/activating transcription factor site and the TATA box of the human TGF-beta2 promoter. By site-directed mutagenesis, we demonstrate that this E-box motif is necessary for the promoter activity, not only in differentiated cells derived from embryonal carcinoma cells, but also in choriocarcinoma cells and in MCF-7 breast carcinoma cells. We also demonstrate that the transcription factors USF1 and USF2 bind to this E-box motif in vitro when nuclear extracts from each of these cell lines are examined by gel retardation assays. Moreover, using a dominant-negative USF2 protein, we show that USF proteins are critical for TGF-beta2 promoter activity in vivo. The importance of the E-box motif described in this study is supported by the presence of an E-box motif in the same position in the chicken TGF-beta2 gene promoter.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Biological Chemistry. - 271 : 50 (1996), p. 32375-32380. -
További szerzők:	Kingsley-Kallesen, Michelle Rizzino, Angie
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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