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001-es BibID:	BIBFORM029806
Első szerző:	Alexy Tamás
Cím:	Effect of lanthanides on red blood cell deformability and response to mechanical stress : role of lanthanide ionic radius / Tamas Alexy, Oguz K. Baskurt, Norbert Nemeth, Mehmet Uyuklu, Rosalinda B. Wenby, Herbert J. Meiselman
Dátum:	2011
ISSN:	0006-355X
Megjegyzések:	Prior studies exploring the effects of lanthanides (Ln) on red blood cells (RBC) have primarily focused on ion transport, cell fusion, and membrane protein structure. Our previous report [Biorheology 44 (2007), 361-373] dealt only with lanthanum (La) and cell rigidity; the present study extends these observations to other lanthanides (Nd, Sm, Eu, Dy, Er) and to RBC response to mechanical shear. Deformation-shear stress behavior of normal human RBC was measured at Ln concentrations up to 200 [mű]M. In another series of experiments, RBC were exposed to mechanical stress (190 Pa, 300 s) at 50 [mű]M Ln and deformation-stress data obtained prior to and after this stress. Data were fitted to a Lineweaver-Burke model to obtain the shear stress at one-half maximum deformation (SS1/2). Our results include: (1) lanthanides cause decreased cell deformability with the magnitude of the decrease dependent on concentration and shear stress; (2) this decrease of deformability is affected by Ln ionic radius such that La>Nd>Sm>Eu>Dy>Er and is reversible for cells in Ln-free media; (3) mechanical stress decreases deformability (i.e., increases SS1/2) such that compared to control, La and Sm reduce and Dy and Er enhance the mechanical stress effect; (4) the decrease of deformability consequent to mechanical stress scales inversely with Ln ionic radius. These results indicate a reciprocal relation between cell rigidity and sensitivity to mechanical stress that is mediated by Ln ionic radius. Additional studies are clearly warranted, particularly those that explore membrane-glycocalyx and intracellular mechanisms.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Biorheology 48 : 3-4 (2011), p. 173-183. -
További szerzők:	Baskurt, Oguz K. Németh Norbert (1975-) (kutatóorvos) Uyuklu, Mehmet Wenby, Rosalinda B. Meiselman, Herbert J.
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM003718
Első szerző:	Alexy Tamás
Cím:	Effect of lanthanum on red blood cell deformability / Alexy, T., Németh, N., Wenby, R. B., Bauersachs, R. M., Baskurt, O. K., Meiselman, H. J.
Dátum:	2007
Megjegyzések:	Prior reports describing the effects of lanthanum (La(3+)) on red blood cells (RBC) have focused on the effects of this lanthanide on cell fusion or on membrane characteristics (e.g., ion movement across membrane, membrane protein aggregation); the present study explores its rheological and biophysical effects. Normal human RBC were exposed to La(3+) levels up to 200 microM then tested for: (1) cellular deformability using a laser-based ektacytometer and an optical-based rheoscope; (2) membrane viscoelastic behavior via micropipettes; (3) surface charge via micro electrophoresis. La(3+) concentrations of 12.5 to 200 microM caused dose-dependent decreases of deformability that were greatest at low stresses: these rheological changes were completely reversible upon removing La(3+) from the media either by washing with La(3+)-free buffer or by suspending La(3+)-exposed cells in La(3+)-free media (i.e., viscous dextran solution). Both membrane shear elastic modulus and membrane surface viscosity were increased by 25-30% at 100 or 200 microM. As expected, La(3+) decreased RBC electrophoretic mobility (EPM), with EPM inversely but not linearly associated with deformability; changes of EPM were also completely reversible. These results thus indicate novel aspects of RBC cellular and membrane rheological behavior yet raise questions regarding specific mechanisms responsible for La(3+)-induced alterations.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Biorheology. - 44 : 5-6 (2007), p. 361-373. -
További szerzők:	Németh Norbert (1975-) (kutatóorvos) Wenby, Rosalinda B. Bauersachs, Rupert M. Baskurt, Oguz K. Meiselman, Herbert J.
Internet cím:	elektronikus változat
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