Összesen 2 találat.


001-es BibID:BIBFORM096333
035-os BibID:(cikkazonosító)746 (WoS)000700957900001 (Scopus)85115312388
Első szerző:Imre Alexandra (1992-)
Cím:Virulence Factors and in-Host Selection on Phenotypes in Infectious Probiotic Yeast Isolates (Saccharomyces 'boulardii') / Alexandra Imre, Renátó Kovács, Kitti Pázmándi, Dániel Nemes, Ágnes Jakab, Tünde Fekete, Hanna Viktória Rácz, Ilona Dóczi, Ildikó Bácskay, Attila Gácser, Károly Kovács, László Majoros, Zoltán Farkas, István Pócsi, Walter P. Pfliegler
Megjegyzések:Saccharomyces yeast probiotics (S. 'boulardii') have long been applied in the treatment of several gastrointestinal conditions. Despite their widespread use, they are rare opportunistic path-ogens responsible for a high proportion of Saccharomyces mycosis cases. The potential virulence at-tributes of S. 'boulardii' as well as its interactions with the human immune system have been studied, however, no information is available on how these yeasts may change due to in-host evolution. To fill this gap, we compared the general phenotypic characteristics, cell morphology, virulence factors, epithelial and immunological interactions, and pathogenicity of four probiotic product samples, two mycosis, and eight non-mycosis samples of S. 'boulardii'. We assessed the characteristics related to major steps of yeast infections. Mycosis and non-mycosis isolates both displayed novel characters when compared to the product isolates, but in the case of most virulence factors and in pathogenic-ity, differences were negligible or, surprisingly, the yeasts from products showed elevated levels. No isolates inflicted considerable damage to the epithelial model or bore the hallmarks of immune evasion. Our results show that strains in probiotic products possess characteristics that enable them to act as pathogens upon permissive conditions, and their entry into the bloodstream is not due to active mechanisms but depends on the host. Survival in the host is dependent on yeast phenotypic characteristics which may change in many ways once they start evolving in the host. These facts call attention to the shortcomings of virulence phenotyping in yeast research, and the need for a more thorough assessment of probiotic use.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Saccharomyces boulardii
opportunistic pathogen
Megjelenés:Journal of Fungi. - 7 : 9 (2021), p. 1-29. -
További szerzők:Kovács Renátó László (1987-) (molekuláris biológus) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus) Nemes Dániel (1993-) (gyógyszerész) Jakab Ágnes (1987-) (biológus) Fekete Tünde (1984-) (immunológus, molekuláris biológus, mikrobiológus) Rácz Hanna Viktória (1994-) (biológus) Dóczi Ilona Bácskay Ildikó (1969-) (gyógyszerész, gyógyszertechnológus) Gácser Attila Kovács Károly Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Farkas Zoltán Pócsi István (1961-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:FK-138910
Internet cím:Szerző által megadott URL
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001-es BibID:BIBFORM070028
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Commercial strain-derived clinical Saccharomyces cerevisiae can evolve new phenotypes without higher pathogenicity / Walter P. Pfliegler, Enikő Boros, Kitti Pázmándi, Ágnes Jakab, Imre Zsuga, Renátó Kovács, Edit Urbán, Zsuzsa Antunovics, Attila Bácsi, Matthias Sipiczki, László Majoros, István Pócsi
Megjegyzések:Scope: Saccharomyces cerevisiae is one of the most important microbes in food industry, but there is growing evidence on its potential pathogenicity as well. Its status as a member of human mycobiome is still not fully understood.Methods and results: In this study, we characterise clinical S. cerevisiae isolates from Hungarian hospitals along with commercial baking and probiotic strains, and determine their phenotypic parameters, virulence factors, interactions with human macrophages, and pathogenicity. Four of the clinical isolates could be traced back to commercial strains based on genetic fingerprinting. Our observations indicate that the commercial-derived clinical isolates have evolved new phenotypes and show similar, or in two cases, significantly decreased pathogenicity. Furthermore, immunological experiments revealed that the variability in human primary macrophage activation after co-incubation with yeasts is largely donor- and not isolate-dependent.Conclusion: Isolates in this study offer an interesting insight into the potential microevolution of probiotic and food strains in human hosts. These commensal yeasts display various changes in their phenotypes, indicating that the colonization of the host does not necessarily impose a selective pressure towards higher virulence/pathogenicity.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
baking yeast
Megjelenés:Molecular Nutrition & Food Research 61 : 11 (2017), p. 1601099-1 - 1601099-8. -
További szerzők:Boros Enikő (1989-) (molekuláris biológus) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus) Jakab Ágnes (1987-) (biológus) Zsuga Imre Kovács Renátó László (1987-) (molekuláris biológus) Urbán Edit Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Bácsi Attila (1967-) (immunológus) Sipiczki Mátyás (1948-) (biológus) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Pócsi István (1961-) (vegyész)
Pályázati támogatás:MTA posztdoktori program
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
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