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001-es BibID:	BIBFORM040288
Első szerző:	Gonda Sándor (gyógyszerész)
Cím:	Filamentous fungi from Plantago lanceolata L. leaves : Contribution to the pattern and stability of bioactive metabolites / Sándor Gonda, Attila Kiss, Tamás Emri, Gyula Batta, Gábor Vasas
Dátum:	2013
ISSN:	0031-9422
Megjegyzések:	The aim of this study was to test contribution of plant-associated microorganism (PAMs) to metabolite stability/instability in a medicinal plant matrix.Therefore, PAM strains were isolated and identified based on relevant DNA sequences from Plantago lanceolata leaves. Sterile water extracts of P. lanceolata were incubated with the isolated strains and antioxidants (ascorbic acid (AA), and EDTA) for 15 days, and changes in the concentrations of chief bioactive constituents (aucubin, catalpol, acteoside (=verbascoside)) were quantified by capillary electrophoresis. Phenolic breakdown-products were identified by GC-MS.PAMs were identified from the genera Epicoccum, Bipolaris, Cladosporium, Leptosphaerulina, Aspergillus, Eurotium and Penicillium (pathongens, endophytes, and other species). Some fungi caused significant decomposition of the chief constituents (p < 0.001). Surprisingly, some strains inhibited breakdown of acteoside (p < 0.001). Meanwhile, concentration of several phenolic acids increased in fungi-infested extracts (p < 0.001). Gentisic acid, 4-hydroxyphenyl acetic acid, 4-hydroxybenzoic acid and hydroxytyrosol were only present when the extract was infested with a PAM. The products are powerful antioxidants and chelators. Concentrations of phenolic acids influenced acteoside stability significantly (p < 0.01), as shown by basic data-mining techniques. AA and EDTA also significantly inhibited acteoside breakdown in sterile model solutions (p < 0.05).Our results suggest that the phenolic acid mixture (produced during the fungal proliferation) protected acteoside from breakdown, possibly via its antioxidant activity and metal complexing ability. It was shown that PAMs can increase or decrease the stability of chief metabolites in herbal matrices, and can significantly alter the chemical pattern of the plant matrix.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Plantago lanceolata L. Ribwort plantain Endophyte Plant-associated microorganism Metabolite stability Phenolic acids Acteoside
Megjelenés:	Phytochemistry. - 86 (2013), p. 127-136. -
További szerzők:	Kiss-Szikszai Attila (1975-) (vegyész) Emri Tamás (1969-) (biológus) Batta Gyula (1953-) (molekula-szerkezet kutató) Vasas Gábor (1975-) (biológus-vegyész)
Pályázati támogatás:	K81370 OTKA F046493 OTKA GVOP-3.2.1.-2004-04-0110/3.0 Egyéb GVOP-TST-3.3.1-05/1-2005-05-0004/3.0 Egyéb TÁMOP-4.2.2-08/1-2008-0014 TÁMOP
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001-es BibID:	BIBFORM065270
Első szerző:	Spitzmüller Zsolt (molekuláris biológus)
Cím:	Characterization of extracellular [gamma]-glutamyl transpeptidase from Aspergillus nidulans / Zsolt Spitzmüller, Sándor Gonda, Attila Kiss-Szikszai, Gábor Vasas, István Pócsi, Tamás Emri
Dátum:	2016
ISSN:	1340-3540 1618-2545
Megjegyzések:	Aspergillus nidulans [gamma]-glutamyl transpeptidase (An[gamma]GT, EC 2.3.2.2) was partially purified from the fermentation broth of carbon stressed cultures. Its temperature and pH optimum was 45 ?C and pH 8.0, respectively. An[gamma]GT had little hydrolase activity. It utilized Gln, glutathione and less efficiently oxidized glutathione as [gamma]-glutamyl donors (beside of [gamma]-glutamyl-p-nitroanilide) and amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly) but not hydroxylamine as [gamma]-glutamyl acceptors. We propose that the function of this enzyme is not to degrade, but to produce, [gamma]-glutamyl compounds which may be related to the utilization of extracellular peptides and amino-acids in carbon stressed cultures.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Mycoscience. - 57 : 6 (2016), p. 400-403. -
További szerzők:	Gonda Sándor (1984-) (gyógyszerész) Kiss-Szikszai Attila (1975-) (vegyész) Vasas Gábor (1975-) (biológus-vegyész) Pócsi István (1961-) (vegyész) Emri Tamás (1969-) (biológus)
Pályázati támogatás:	OTKA K100464 OTKA OTKA K112181 OTKA SROP-4.2.2.B-15/1/KONV-2015-0001 egyéb
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001-es BibID:	BIBFORM074731
035-os BibID:	(WoS)000432283600002 (Scopus)85046668578
Első szerző:	Szűcs Zsolt (gyógyszerész)
Cím:	Endophytic fungi from the roots of horseradish (Armoracia rusticana) and their interactions with the defensive metabolites of the glucosinolate - myrosinase - isothiocyanate system / Szűcs Zsolt, Plaszkó Tamás, Cziáky Zoltán, Kiss-Szikszai Attila, Emri Tamás, Bertóti Regina, Sinka László Tamás, Vasas Gábor, Gonda Sándor
Dátum:	2018
ISSN:	1471-2229
Megjegyzések:	AbstractBackgroundThe health of plants is heavily influenced by the intensively researched plant microbiome. The microbiome has to cope with the plant's defensive secondary metabolites to survive and develop, but studies that describe this interaction are rare. In the current study, we describe interactions of endophytic fungi with a widely researched chemical defense system, the glucosinolate - myrosinase - isothiocyanate system. The antifungal isothiocyanates are also of special interest because of their beneficial effects on human consumers.ResultsSeven endophytic fungi were isolated from horseradish roots (Armoracia rusticana), from the genera Fusarium, Macrophomina, Setophoma, Paraphoma and Oidiodendron. LC-ESI-MS analysis of the horseradish extract incubated with these fungi showed that six of seven strains could decompose different classes of glucosinolates. Aliphatic, aromatic, thiomethylalkyl and indolic glucosinolates were decomposed by different strains at different rates. SPME-GC-MS measurements showed that two strains released significant amounts of allyl isothiocyanate into the surrounding air, but allyl nitrile was not detected. The LC-ESI-MS analysis of many strains' media showed the presence of allyl isothiocyanate - glutathione conjugate during the decomposition of sinigrin. Four endophytic strains also accepted sinigrin as the sole carbon source. Isothiocyanates inhibited the growth of fungi at various concentrations, phenylethyl isothiocyanate was more potent than allyl isothiocyanate (mean IC50 was 2.30-fold lower).As a control group, ten soil fungi from the same soil were used. They decomposed glucosinolates with lower overall efficiency: six of ten strains had insignificant or weak activities and only three could use sinigrin as a carbon source. The soil fungi also showed lower AITC tolerance in the growth inhibition assay: the median IC50 values were 0.1925 mM for endophytes and 0.0899 mM for soil fungi.ConclusionsThe host's glucosinolates can be used by the tested endophytic fungi as nutrients or to gain competitive advantage over less tolerant species. These activities were much less apparent among the soil fungi. This suggests that the endophytes show adaptation to the host plant's secondary metabolites and that host metabolite specific activities are enriched in the root microbiome. The results present background mechanisms enabling an understanding of how plants shape their microbiome.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Myrosinase Endophytes Glucosinolate Isothiocyanate Fungal enzyme
Megjelenés:	BMC Plant Biology. - 18 : 1 (2018), p. 1-15. -
További szerzők:	Plaszkó Tamás (1994-) (Biológus Msc) Cziáky Zoltán Kiss-Szikszai Attila (1975-) (vegyész) Emri Tamás (1969-) (biológus) Bertóti Regina Sinka László Tamás Vasas Gábor (1975-) (biológus-vegyész) Gonda Sándor (1984-) (gyógyszerész)
Pályázati támogatás:	112374 OTKA 124339 OTKA EFOP-3.6.1-16-2016-00022 EFOP
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