CCL

Összesen 4 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM029094
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Expression of hyperpolarization-activated and cyclic nucleotide-gated cation channel subunit 2 in axon terminals of peptidergic nociceptive primary sensory neurons in the superficial spinal dorsal horn of rats / Antal, M., Papp, I., Bahaerguli, N., Veress, G., Vereb, G.
Dátum:2004
Megjegyzések:Hyperpolarization-activated cyclic nucleotide-gated cation channel proteins (HCN1-4), which are potentially able to modulate membrane excitability, are abundantly expressed by neurons in spinal dorsal root ganglia (DRG). In the present experiment, we investigated whether HCN2 protein is confined exclusively to the perikarya of DRG neurons or is transported from the somata to the central axons of DRG neurons that terminate in the spinal dorsal horn. Using immunohistochemical methods, we have demonstrated that laminae I-IIo of the superficial spinal dorsal horn of the adult rat spinal cord show a strong punctate immunoreactivity for HCN2. Dorsal rhizotomy resulted in a complete loss of immunostaining in the dorsal horn, suggesting that HCN2 is confined to axon terminals of primary afferents. In double labelling immunohistochemical studies, we have also shown that HCN2 widely co-localizes with calcitonin gene-related peptide, but is almost completely segregated from isolectin-B4 binding, indicating that HCN2 is primarily expressed in peptidergic nociceptive primary afferents. The expression of HCN2 in central terminals of peptidergic primary afferents was also verified with electron microscopy. Utilizing the pre-embedding nanogold method, we found that HCN2 is largely confined to axon terminals with dense-core vesicles. Within these terminals, some of the silver grains marking the accurate location of HCN2 molecules were associated with the cell membrane, and others were scattered in the axoplasm. Within the cell membrane, HCN2 was found almost exclusively in extrasynaptic locations. The results suggest that HCN2 may contribute to the modulation of membrane excitability of nociceptive primary afferent terminals in the spinal dorsal horn.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The European Journal of Neuroscience 19 : 5 (2004), p. 1336-1342. -
További szerzők:Papp Ildikó (1976-) (biológus) Bahaerguli, Niyazi Veress Gábor (1971-) (neurobiológus) Vereb György (1965-) (biofizikus, orvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
elektronikus változat
DOI
Borító:

2.

001-es BibID:BIBFORM069841
Első szerző:Holló Krisztina (vegyész)
Cím:Interleukin-1 receptor type 1 is over-expressed in neurons but not in glial cells within the rat superficial spinal dorsal horn in complete Freund adjuvant induced inflammatory pain / Krisztina Holló, László Ducza, Zoltán Hegyi, Klaudia Dócs, Krisztina Hegedűs, Erzsébet Bakk, Ildikó Papp, Gréta Kis, Zoltán Mészár, Zsuzsanna Bardóczi, Miklós Antal
Dátum:2017
Megjegyzések:AbstractBackground: All known biological functions of the pro-inflammatory cytokine interleukin-1? (IL-1?) are mediatedby type 1 interleukin receptor (IL-1R1). IL-1??IL-1R1 signaling modulates various neuronal functions including spinalpain processing. Although the role of IL-1? in pain processing is generally accepted, there is a discussion in the literaturewhether IL-1? exerts its effect on spinal pain processing by activating neuronal or glial IL-1R1. To contributeto this debate, here we investigated the expression and cellular distribution of IL-1R1 in the superficial spinaldorsal horn in control animals and also in inflammatory pain.Methods: Experiments were performed on rats and wild type as well as IL-1R1-deficient mice. Inflammatorypain was evoked by unilateral intraplantar injection of complete Freund adjuvant (CFA). The nociceptiveresponsiveness of control and CFA-treated animals were tested daily for withdrawal responses to mechanicaland thermal stimuli before and after CFA injection. Changes in the expression of 48 selected genes/mRNAsand in the quantity of IL-1R1 protein during the first 3 days after CFA injection were measured with theTaqMan low-density array method and Western blot analysis, respectively. The cellular localization of IL-1R1protein was investigated with single and double staining immunocytochemical methods.Results: We found a six times and two times increase in IL-1R1 mRNA and protein levels, respectively, in thedorsal horn of CFA-injected animals 3 days after CFA injection, at the time of the summit of mechanical andthermal allodynia. Studying the cellular distribution of IL-1R1, we found an abundant expression of IL-1R1 onthe somatodendritic compartment of neurons and an enrichment of the receptor in the postsynaptic membranes ofsome excitatory synapses. In contrast to the robust neuronal localization, we observed only a moderate expression ofIL-1R1 on astrocytes and a negligible one on microglial cells. CFA injection into the hind paw caused a remarkableincrease in the expression of IL-1R1 in neurons, but did not alter the glial expression of the receptor.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
IL-1R1
Rodents
Inflammatory pain evoked by CFA injection
Immunohistochemistry
Superficial spinal dorsal horn
Megjelenés:Journal of Neuroinflammation. - 14 : 1 (2017), p. 1-18. -
További szerzők:Ducza László (1987-) (molekuláris biológus) Hegyi Zoltán (1983-) (molekuláris biológus) Dócs Klaudia Hegedűs Krisztina Bakk Erzsébet Papp Ildikó (1976-) (biológus) Kis Gréta (1979-) (környezetkutató) Mészár Zoltán Mihály (1977-) (agrármérnök) Bardóczi Zsuzsanna Antal Miklós (1951-) (orvos, anatómus)
Pályázati támogatás:KTIA_NAP_13-1-2013-0001
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM029097
Első szerző:Papp Ildikó (biológus)
Cím:Hyperpolarization-activated and cyclic nucleotide-gated cation channel subunit 2 ion channels modulate synaptic transmission from nociceptive primary afferents containing substance P to secondary sensory neurons in laminae I-IIo of the rodent spinal dorsal horn / Papp I., Szűcs P., Holló K., Erdélyi F., Szabó G., Antal M.
Dátum:2006
Megjegyzések:We have previously demonstrated that hyperpolarization-activated and cyclic nucleotide-gated cation channel subunit 2 (HCN2) is expressed by terminals of peptidergic nociceptive primary afferents in laminae I-IIo of the rat spinal dorsal horn. In this study, we investigated the possible neurotransmitters and postsynaptic targets of these HCN2-expressing primary afferent terminals in the superficial spinal dorsal horn by using immunocytochemical methods. We demonstrated that HCN2 widely colocalizes with substance P (SP), and that HCN2-positive terminals that are also immunoreactive for SP form serial close appositions with dendrites and perikarya of neurokinin 1 receptor-immunoreactive neurons. It was also found that HCN2-immunoreactive terminals are frequently apposed to neurons that are immunoreactive for calbindin, mu-opioid receptor and the alfa-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor subunit GluR2, markers for excitatory interneurons. Investigating HCN2 immunoreactivity in glutamic acid decarboxylase 65-green fluorescent protein transgenic mice, we found that HCN2-positive terminals occasionally also contact cells that contain an isoform of glutamic acid decarboxylase (glutamic acid decarboxylase 65), a marker for GABAergic inhibitory neurons. Application of ZD7288, an antagonist of HCN channels, onto neurons that were recorded in spinal cord slices with whole-cell patch-clamp electrodes reduced the number of monosynaptic excitatory postsynaptic potentials evoked by electrical stimulation of primary afferents at nociceptive intensities. The results suggest that HCN2 may contribute to the modulation of membrane excitability of SP-containing nociceptive primary afferent terminals, may increase the reliability of synaptic transmission from primary afferents to secondary sensory neurons and thus may play a role in the fine-tuning of pain transmission from nociceptive primary afferents to neurons in the spinal dorsal horn.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:European Journal of Neuorscience. - 24 : 5 (2006), p. 1341-1352. -
További szerzők:Szűcs Péter (1974-) (kutatóorvos) Holló Krisztina (1967-) (vegyész) Erdélyi Ferenc Szabó Gábor (budapesti orvos) Antal Miklós (1951-) (orvos, anatómus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

4.

001-es BibID:BIBFORM013294
Első szerző:Papp Ildikó (biológus)
Cím:Plasticity of hyperpolarization-activated and cyclic nucleotid-gated cation channel subunit 2 expression in the spinal dorsal horn in inflammatory pain / Ildikó Papp, Krisztina Holló, Miklós Antal
Dátum:2010
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Egészség- és Környezettudomány
Megjelenés:The European Journal of Neuroscience. - 32 : 7 (2010), p. 1193-1201. -
További szerzők:Holló Krisztina (1967-) (vegyész) Antal Miklós (1951-) (orvos, anatómus)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
A gerincvelő felületes hátsó szarvi neuronhálózatok szerveződése és plaszticitása krónikus gyulladásos és neuropátiás fájdalom állapotokban
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
elektronikus változat
Borító:
Rekordok letöltése1