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001-es BibID:BIBFORM118410
035-os BibID:(cikkazonosító)2300931 (Scopus)85168319047 (WOS)001049575600001
Első szerző:Gao, Liang
Cím:A Photopolymerizable Biocompatible Hyaluronic Acid Hydrogel Promotes Early Articular Cartilage Repair in a Minipig Model In Vivo / Liang Gao, Riccardo Beninatto, Tamás Oláh, Lars Goebel, Ke Tao, Rebecca Roels, Steffen Schrenker, Julianne Glomm, Jagadeesh K. Venkatesan, Gertrud Schmitt, Ebrar Sahin, Ola Dahhan, Mauro Pavan, Carlo Barbera, Alba Di Lucia, Michael D. Menger, Matthias W. Laschke, Magali Cucchiarini, Devis Galesso, Henning Madry
Dátum:2023
ISSN:2192-2640 2192-2659
Megjegyzések:Articular cartilage defects represent an unsolved clinical challenge. Photopolymerizable hydrogels are attractive candidates supporting repair. This study investigates the short-term safety and efficacy of two novel hyaluronic acid (HA)-triethylene glycol (TEG)-coumarin hydrogels photocrosslinked in situ in a clinically relevant large animal model. It is hypothesized that HA-hydrogel-augmented microfracture (MFX) is superior to MFX in enhancing early cartilage repair, and that the molar degree of substitution and concentration of HA affects repair. Chondral full-thickness defects in the knees of adult minipigs are treated with either 1) debridement (No MFX), 2) debridement and MFX, 3) debridement, MFX, and HA hydrogel (30% molar derivatization, 30 mg mL?1 HA; F3) (MFX+F3), and 4) debridement, MFX, and HA hydrogel (40% molar derivatization, 20 mg mL?1 HA; F4) (MFX+F4). After 8 weeks postoperatively, MFX+F3 significantly improves total macroscopic and histological scores compared with all other groups without negative effects, besides significantly enhancing the individual repair parameters "defect architecture," "repair tissue surface" (compared with No MFX, MFX), and "subchondral bone" (compared with MFX). These data indicate that photopolymerizable HA hydrogels enable a favorable metastable microenvironment promoting early chondrogenesis in vivo. This work also uncovers a mechanism for effective HA-augmented cartilage repair by combining lower molar derivatization with higher concentrations.
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
cartilage defects
cartilage repairs
hyaluronic acid
hydrogels
large animal model
photo-crosslinkin
Megjelenés:Advanced Healthcare Materials. - 12 : 26 (2023), p. 2300931. -
További szerzők:Beninatto, Riccardo Oláh Tamás (1983-) (élettanász) Goebel, Lars Tao, Ke Roels, Rebecca Schrenker, Steffen Glomm, Julianne Venkatesan, Jagadeesh K. Schmitt, Gertrud Sahin, Ebrar Dahhan, Ola Pavan, Mauro Barbera, Carlo Lucia, Alba Di Menger, Michael D. Laschke, Matthias W. Cucchiarini, Magali Galesso, Devis Madry, Henning
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2.

001-es BibID:BIBFORM115442
035-os BibID:(WoS)000469480000024 (Scopus)85053303109
Első szerző:Gao, Liang
Cím:Asymptomatic focal calcium pyrophosphate crystal deposition within partially failed repair tissue after matrix-assisted autologous chondrocyte implantation / Liang Gao, Tamás Oláh, Magali Cucchiarini, Henning Madry
Dátum:2018
ISSN:0942-2056
Megjegyzések:Possible failures of autologous chondrocyte implantation (ACI), a cell-based technique for articular cartilage repair, are not always clinically apparent and the underlying mechanisms largely remain unknown. This case report presents the first scenario in the literature highlighting an association of a medium-term partial failure of an advanced ACI procedure (matrix-assisted ACI) in the knee with focal asymptomatic calcium pyrophosphate deposition disease, a common inflammatory pyrophosphate arthropathy. The specific presence of CPPDs, resulting from increased biomechanical stresses in the repair tissue?cartilage and repair tissue?subchondral bone integration sites, together with the absence of cartilage regeneration was identified and possibly contributed to the partial failure.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Matrix-assisted autologous chondrocyte implantation
Calcium pyrophosphate deposition disease
Asymptomatic failure
Mid-term outcome
Knee
Megjelenés:Knee Surgery Sports Traumatology Arthroscopy. - 27 : 6 (2018), p. 1939-1942. -
További szerzők:Oláh Tamás (1983-) (élettanász) Cucchiarini, Magali Madry, Henning
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3.

001-es BibID:BIBFORM115440
035-os BibID:(Scopus)85040458576 (WOS)000419442500012 (cikkazonosító)75
Első szerző:Oláh Tamás (élettanász)
Cím:Reliable landmarks for precise topographical analyses of pathological structural changes of the ovine tibial plateau in 2-D and 3-D subspaces / Tamás Oláh, Jan Reinhard, Liang Gao, Lars K. H. Goebel, Henning Madry
Dátum:2018
ISSN:2045-2322
Megjegyzések:Selecting identical topographical locations to analyse pathological structural changes of the osteochondral unit in translational models remains difficult. The specific aim of the study was to provide objectively defined reference points on the ovine tibial plateau based on 2-D sections of micro-CT images useful for reproducible sample harvesting and as standardized landmarks for landmark-based 3-D image registration. We propose 5 reference points, 11 reference lines and 12 subregions that are detectable macroscopically and on 2-D micro-CT sections. Their value was confirmed applying landmark-based rigid and affine 3-D registration methods. Intra- and interobserver comparison showed high reliabilities, and constant positions (standard errors?<?1%). Spatial patterns of the thicknesses of the articular cartilage and subchondral bone plate were revealed by measurements in 96 individual points of the tibial plateau. As a case study, pathological phenomena 6 months following OA induction in vivo such as osteophytes and areas of OA development were mapped to the individual subregions. These new reference points and subregions are directly identifiable on tibial plateau specimens or macroscopic images, enabling a precise topographical location of pathological structural changes of the osteochondral unit in both 2-D and 3-D subspaces in a region-appropriate fashion relevant for translational investigations.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Scientific Reports. - 8 : 1 (2018), p. 75. -
További szerzők:Reinhard, Jan Gao, Liang Goebel, Lars Madry, Henning
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4.

001-es BibID:BIBFORM115267
035-os BibID:(Scopus)85071741811 (WoS)000484406300004 (cikkazonosító)eaax6775
Első szerző:Oláh Tamás (élettanász)
Cím:Topographic modeling of early human osteoarthritis in sheep / Tamás Oláh, Jan Reinhard, Liang Gao, Sophie Haberkamp, Lars K. H. Goebel, Magali Cucchiarini, Henning Madry
Dátum:2019
ISSN:1946-6234
Megjegyzések:Articular cartilage damage occurring during early osteoarthritis (OA) is a key event marking the development of the disease. Here, we modeled early human OA by gathering detailed spatiotemporal data from surgically induced knee OA development in sheep. We identified a specific topographical pattern of osteochondral changes instructed by a defined meniscal injury, showing that both cartilage and subchondral bone degeneration are initiated from the region adjacent to the damage. Alterations of the subarticular spongiosa arising locally and progressing globally disturbed the correlations of cartilage with subchondral bone seen at homeostasis and were indicative of disease progression. We validated our quantitative findings against human OA, showing a similar pattern of early OA correlating with regions of meniscal loss and an analogous late critical disturbance within the entire osteochondral unit. This translational model system can be used to elucidate mechanisms of OA development and provides a roadmap for investigating regenerative therapies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Science Translational Medicine. - 11 : 508 (2019), p. eaax6775. -
További szerzők:Reinhard, Jan Gao, Liang Haberkamp, Sophie Goebel, Lars Cucchiarini, Magali Madry, Henning
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5.

001-es BibID:BIBFORM114887
035-os BibID:(WOS)000806728200001 (Scopus)85131298379 (cikkazonosító)2201692
Első szerző:Oláh Tamás (élettanász)
Cím:Quantifying the Human Subchondral Trabecular Bone Microstructure in Osteoarthritis with Clinical CT / Tamás Oláh, Xiaoyu Cai, Liang Gao, Frédéric Walter, Dietrich Pape, Magali Cucchiarini, Henning Madry
Dátum:2022
ISSN:2198-3844
Megjegyzések:Osteoarthritis (OA) is characterized by critical alterations of the subchondral bone microstructure, besides the well-known cartilaginous changes. Clinical computed tomography (CT) detection of quantitative 3D microstructural subchondral bone parameters is applied to monitor changes of subchondral bone structure in different stages of human OA and is compared with micro-CT, the gold standard. Determination by clinical CT (287 ?m resolution) of key microstructural parameters in tibial plateaus with mild-to-moderate and severe OA reveals strong correlations to micro-CT (35 ?m), high inter- and intraobserver reliability, and small relative differences. In vivo, normal, mild-to-moderate, and severe OA are compared with clinical CT (331 ?m). All approaches detect characteristic expanded trabecular structure in severe OA and fundamental microstructural correlations with clinical OA stage. Multivariate analyses at various in vivo and ex vivo imaging resolutions always reliably separate mild-to-moderate from severe OA (except mild-to-moderate OA from normal), revealing a striking similarity between 287 ?m clinical and 35 ?m micro-CT. Thus, accurate structural measurements using clinical CT with a resolution near the trabecular dimensions are possible. Clinical CT offers an opportunity to quantitatively monitor subchondral bone microstructure in clinical and experimental settings as an advanced tool of investigating OA and other diseases affecting bone architecture.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
3D microstructure
computed tomography
microcomputed tomography
multislice computed tomography
osteoarthritis of knee
subchondral bone
Megjelenés:Advanced Science. - 9 : 23 (2022), p. 2201692. -
További szerzők:Cai, Xiaoyu Gao, Liang Walter, Frédéric Pape, Dietrich Cucchiarini, Magali Madry, Henning
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