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001-es BibID:	BIBFORM056052
Első szerző:	Demeter Zita (biológia-kémia szakos tanár)
Cím:	Osmotic stress responses of individual white oak (Quercus section, Quercus subgenus) genotypes cultured in vitro / Zita Demeter, Péter Kanalas, Csaba Máthé, Klára Cseke, Erzsébet Szőllősi, Márta M-Hamvas, Katalin Jámbrik, Zoltán Kiss, Ilona Mészáros
Dátum:	2014
ISSN:	0176-1617
Megjegyzések:	White oaks (Quercus section, Quercus subgenus) are widely distributed in Europe. Quercus petraea (sessile oak), an economically important species is predicted to be affected by climate change. Q. pubescens (pubescent oak) and Q. virgiliana (Italian pubescent oak) are economically less important, drought tolerant species. Frequent hybridization of white oaks was observed and currently the introgression of Q. pubescens and Q. virgiliana in non-mediterranean regions of Europe has been reported. Our goal was to use tissue cultures established from individual trees of the above taxa and their putative hybrids, all present in the forest stand of Síkfőkút LTER Research Area (NE Hungary) as simple experimental model systems for studying drought/osmotic stress tolerance. Tissue cultures are more suitable models for such studies, than seedlings, because they are genetically identical to the parent plants. Polyethylene glycol (PEG6000) treatments were used for this purpose. The identification of taxa was based on leaf morphological traits and microsatellite analysis and showed that Q. petraea is genetically distinct to all other taxa examined. We established six callus lines of Quercus. As expected, in Q. petraea cultures PEG6000 induced severe loss of fresh weight and the ability to recover after removal of the osmoticum, which was not characteristic for Q. pubescens and Q. virgiliana. Putative hybrids exhibited an intermediate response to osmotic stress. Activity gels showed the increase of single-strand preferring (SSP) nuclease and no significant change of guaiacol-peroxidase activities in drought-sensitive genotypes/cultures and no significant increase of SSP nuclease activities accompanied with increases of guaiacol-peroxidase activities in drought-tolerant ones. This indicates that drought/osmotic stress tolerance is associated to increased capacity of scavenging reactive oxygen species and hence less susceptibility to DNA damage. Our results confirm that tissue cultures of oak are suitable model systems for studying drought/osmotic stress responses.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Quercus Osmotic Stress in vitro
Megjelenés:	Journal Of Plant Physiology. - 171 : 2 (2014), p. 16-24. -
További szerzők:	Kanalas Péter (1983-) (biológus) Máthé Csaba (1966-) (biológus) Cseke Klára Szőllősi Erzsébet (1983-) (biológus) Mikóné Hamvas Márta (1963-) (biológus) Jámbrik Katalin Kiss Zoltán Mészáros Ilona (1952-) (biológus)
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001-es BibID:	BIBFORM056117
Első szerző:	Máthé Csaba (biológus)
Cím:	The plant tissue culture collection at the Department of Botany, University of Debrecen / Csaba Máthé, Zita Demeter, Anna Resetár, Sándor Gonda, Andrea Balázs, Éva Szőke, Zoltán Kiss, Ádám Simon, Viktória Székely, Milán Riba, Tamás Garda, Boglárka Gere, Zsófia Noszály, Attila Molnár V., Gábor Vasas
Dátum:	2012
ISSN:	1588-385X
Megjegyzések:	We present a list of tissue culture systems developed at the Department of Botany,University of Debrecen. Several of tissue cultures were developed for the first time in our laboratory.These include micropropagation of Elatine hungarica, callus cultures of several oak genotypesof Hungarian origin and of Crocus species characteristic for the Carpathian Basin. Calluscultures were either organogenic (e.g. Crocus scepusiensis) or embryogenic (Quercus petraea,Galanthus and Crocus species, Phragmites australis). In case of embryogenic cultures, somaticembryos showed either normal, bipolar development (e.g. Crocus heuffelianus, Phragmitesaustralis) or they have lost this bipolarity at the maturation of embryos (C. banaticus, C. sativus,Galanthus). The type of callus is yet to be identified for several cultures (e.g. Plantago lanceolata,Vicia faba). Most of our in vitro cultures proved to have plant regeneration potential. Severalof them derived from endangered/ red list plants (Crocus species, Elatine hungarica, Galanthusnivalis), therefore they are suitable for germplasm preservation. Others (Quercus, Phragmitesaustralis) proved to be suitable for stress physiology and/ or cell biology experiments. Culturessuch as Crocus sativus or Plantago lanceolata derived from plants of medicinal importance,therefore are of potential pharmacological use.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény hazai lapban tissue culture germplasm preservation embryogenic callus organogenesis micropropagation
Megjelenés:	Acta Biologica Szegediensis. - 56 : 2 (2012), p. 179-182. -
További szerzők:	Demeter Zita (1985-) (biológia-kémia szakos tanár) Resetár Anna Gonda Sándor (1984-) (gyógyszerész) Balázs Andrea Szőke Éva Kiss Zoltán Simon Ádám (1990-) (molekuláris biológus) Székely Viktória Riba Milán Garda Tamás (1990-) (biológus) Gere Boglárka Noszály Zsófia Molnár V. Attila (1969-) (biológus, botanikus) Vasas Gábor (1975-) (biológus-vegyész)
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