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001-es BibID:	BIBFORM073332
Első szerző:	Lotz Gábor
Cím:	Localization of hepatitis C virus RNA on human erythrocytes by RT in situ PCR technique / G. Lotz, F. Szalay, G. Firneisz, M. Abonyi, G. Lengyel, L. Telegdy, E. Ibrányi, B. Nemes, F. Kury, Z. Schaff, S. Simon
Dátum:	2002
ISSN:	0036-5521
Megjegyzések:	Our previous results showed that hepatitis C virus (HCV) is detectable on erythrocytes by RT in situ PCR. The aims of the present study were to compare the sensitivity of this erythrocyte in situ PCR to routine serum solution phase HCV PCR as well as to obtain more data about the binding and cellular localization of HCV in the erythrocyte. METHODS: 105 previously HCV-infected patients and 20 control individuals were studied using RT in situ PCR on erythrocytes and solution phase RT-PCR from serum samples. Binding of HCV to erythrocytes was studied by in vitro inoculation. RT in situ PCR results were evaluated by fluorescence and confocal laser scanning microscopy. RESULTS: From 105 HCV cases, 78 gave positive, while 5-and all control cases-gave negative results by both PCR techniques. In 21 cases, only the in situ technique provided positive results, while in only I case did the solution phase method provide positive results. During in vitro inoculation, an early HCV-erythrocyte binding was detected followed by virus internalization. CONCLUSIONS: Erythrocyte-in situ PCR was found to show higher sensitivity for the detection of HCV compared to the generally applied serum PCR method. In vitro studies suggested a specific binding of HCV to erythrocyte and showed the virus to be capable of internalization.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Scandinavian Journal Of Gastroenterology 37 : 5 (2002), p. 578-584. -
További szerzők:	Szalay Ferenc Firneisz Gábor Tóth-Abonyi Mihály Lengyel Gabriella Telegdy László Ibrányi Endre Nemes Balázs Áron (1969-) (sebész) Kury F. Schaff Zsuzsa Simon, Susan
Pályázati támogatás:	AKP 98-78 3 Egyéb AKP 98-78 2 Egyéb T 023579 OTKA T 029266 OTKA FKFP 0305/1999 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM073336
Első szerző:	Lotz Gábor
Cím:	Detection of chlamydia pneumoniae in liver transplant patients with chronic allograft rejection / Gábor Lotz, Susan Simon, Attila Patonai, Péter Sótonyi, Balázs Nemes, Consolato Sergi, Tibor Glasz, Tibor Füle, Björn Nashan, Zsuzsa Schaff
Dátum:	2004
ISSN:	0041-1337
Megjegyzések:	Chlamydia pneumoniae is one of the possible pathogenetic factors of atherosclerotic processes. Foam cell arteriopathy is a generally accepted pathologic feature of chronic liver allograft rejection and has several similarities to the early lesions of atherosclerosis. The aim of the authors' study was to show any existing correlation between the occurrence of Chlamydia pneumoniae and the presence of foam cell arteriopathy in transplanted livers with chronic rejection. METHODS: Ten liver samples from patients with chronic liver rejection including foam cell arteriopathy and 10 liver samples from healthy individuals were analyzed for the presence of Chlamydia pneumoniae by specific immunohistochemistry and polymerase chain reaction (PCR). Liver samples from two transplant patients with chronic liver rejection without any evidence of foam cell arteriopathy and nine patients with acute liver allograft rejection were also investigated by PCR. RESULTS: In all 10 rejected liver samples, Chlamydia pneumoniae was detected by PCR, whereas only one of the healthy control samples and one of the samples with acute rejection were found to be positive. Immunohistochemistry showed similar results. The positive signals of Chlamydia pneumoniae were localized mainly in the hepatocytes, sinusoidal and perisinusoidal cells, and the cells of portal tracts, whereas most of the altered hepatic arteries showed no or very weak positivity. CONCLUSIONS: The results strongly suggest an association between the occurrence of Chlamydia pneumoniae and the presence of foam cell arteriopathy in transplanted livers.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Transplantation 77 : 10 (2004), p. 1522-1528. -
További szerzők:	Simon, Susan Patonai Attila Sótonyi Péter (1971-) (érsebész) (jr.) Nemes Balázs Áron (1969-) (sebész) Sergi, Consolato Glasz Tibor Füle Tibor Nashan, Björn Schaff Zsuzsa
Pályázati támogatás:	NKFP-1A/0023/2002 NKFP T037838 OTKA
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