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001-es BibID:	BIBFORM033174
Első szerző:	Hevessy Zsuzsanna (laboratóriumi szakorvos)
Cím:	Laboratory evaluation of a flow cytometric BCR-ABL immunobead assay / Zsuzsanna Hevessy, Renáta Hudák, Valéria Kiss-Sziráki, Péter Antal-Szalmás, Miklós Udvardy, László Rejtő, László Szerafin, János Kappelmayer
Dátum:	2011
Megjegyzések:	BACKGROUND: A new flow cytometric (FC) BCR-ABL immunobead assay has been developed recently. Here we present the laboratory evaluation of the commercially available kit. METHODS: Mononuclear cells were isolated, lysed and processed according to the instructions of the manufacturer. Anti-BCR antibodies adsorbed to capture beads bind the BCR-ABL fusion proteins of the lysed cells, a phycoerythrin (PE)-conjugated anti-ABL antibody is the detector reagent and mean fluorescence intensity (MFI) signals were recorded by flow cytometry. Detection of t(9;22)(q34;q11) translocation was carried out with a quantitative PCR assay. RESULTS: MFI results of 20 normal peripheral blood samples were 88±8 (mean±SD), CV 9%. K562 cells were used as positive control. Within-batch imprecision was excellent (3.7% in the normal and 10% in the pathological range). Cut-off was chosen at MFI 112, where both sensitivity and specificity were 100%. Altogether 17 chronic myeloid leukemia (CML) and 16 acute leukemia samples were analyzed. All PCR positive samples (n=14) were positive with the FC method and negative results were also concordant (n=15). Frozen cell lysates can be stored up to 4 weeks without significant decrease of MFI signal. CONCLUSIONS: The FC BCR-ABL assay is a fast, reproducible and reliable method that may be incorporated into standard flow cytometric protocols to help clinical decision-making.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény Molekuláris Medicina
Megjelenés:	Clinical Chemistry and Laboratory Medicine. - 50 : 4 (2011), p. 689-692. -
További szerzők:	Hudák Renáta Kiss-Sziráki Valéria Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Udvardy Miklós (1947-) (belgyógyász, haematológus) Rejtő László (1963-) (belgyógyász, haematológus) Szerafin László (1958-) (belgyógyászat, haematológia, klinikai onkológia szakorvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Celluláris hematológia - immunológia
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM068933
Első szerző:	Hudák Renáta
Cím:	Laboratory characterization of leukemic cell procoagulants / Renáta Hudák, Ildikó Beke Debreceni, Ivett Deák, Gabriella Gál Szabó, Zsuzsanna Hevessy, Péter Antal-Szalmás, Bjarne Osterud, János Kappelmayer
Dátum:	2017
ISSN:	1434-6621
Megjegyzések:	Background: In acute myeloid leukemias, there is anincreased chance to develop thrombotic disorders. Wehypothesized that in addition to leukemic promyelocytes,monocytic leukemia cells may also have a higher procoagulantactivity.Methods: Fibrin formation was assessed by a one-stageclotting assay using a magnetic coagulometer. The thrombingeneration test (TGT) of magnetically isolated normalhuman monocytes, intact leukemic cells and their isolatedmicroparticles was performed by a fluorimetric assay.Phosphatidylserine (PS) expression of leukemic cells andmicroparticle number determinations were carried out byflow cytometry.Results: All cell lines displayed a significant procoagulantpotential compared to isolated normal human monocytes.In the TGT test, the mean of lagtime and the time to peakparameters were significantly shorter in leukemic cells(3.9?4.7 and 9.9?10.3 min) compared to monocytes (14.9and 26.5 min). The mean of peak thrombin in variousmonocytic leukemia cell lines was 112.1?132.9 nM vs.75.1 nM in monocytes; however, no significant differencewas observed in the ETP parameter. Factor VII-deficientplasma abolished all procoagulant activity, whereas factorXII-deficient plasma did not affect the speed of fibrinformation and thrombin generation but modulated theamount of thrombin. Factor XI-deficient plasma affectedthe time to peak values in one leukemic cell line and alsoattenuated peak thrombin. Leukemia cell-derived microparticlesfrom all three cell lines exerted a procoagulanteffect by significantly shortening the lagtime in TGT; therewas a nonsignificant difference in case of ETP parameter.Conclusions: All investigated monocytic leukemia celllines exhibited significant thrombin generation. This phenomenonwas achieved by the procoagulants on the surfaceof leukemic cells as well as by their microparticles.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban monocytic leukemia tissue factor
Megjelenés:	Clinical Chemistry and Laboratory Medicine 55 : 8 (2017), p. 1215-1223. -
További szerzők:	Bekéné Debreceni Ildikó (1970-) (biológus) Deák Ivett Gál Szabó Gabriella Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Osterud, Bjarne Kappelmayer János (1960-) (laboratóriumi szakorvos)
Pályázati támogatás:	TÁMOP-4.2.4.A/2-11-1-2012-0001 TÁMOP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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