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001-es BibID:BIBFORM055415
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Generation of new genotypic and phenotypic features in artificial and natural yeast hybrids / Walter P. Pfliegler, Lea Atanasova, Edina Karanyicz, Matthias Sipiczki, Ursula Bond, Irina S. Druzhinina, Katja Sterflinger, Ksenija Lopandic
ISSN:1330-9862 1334-2606
Megjegyzések:Evolution and genome stabilization have mostly been studied on the Saccharomyceshybrids isolated from natural and alcoholic fermentation environments. Genetic and phenotypicproperties have usually been compared to the laboratory and reference strains, as thetrue ancestors of the natural hybrid yeasts are unknown. In this way the exact impact ofdifferent parental fractions on the genome organization or metabolic activity of the hybridyeasts is difficult to resolve completely. In the present work the evolution of geno- andphenotypic properties is studied in the interspecies hybrids created by the cross-breedingof S. cerevisiae with S. uvarum or S. kudriavzevii auxotrophic mutants. We hypothesized thatthe extent of genomic alterations in S. cerevisiae ? S. uvarum and S. cerevisiae ? S. kudriavzeviishould affect the physiology of their F1 offspring in different ways. Our results, obtainedby amplified fragment length polymorphism (AFLP) genotyping and karyotypinganalyses, showed that both subgenomes of the S. cerevisiae x S. uvarum and of S. cerevisiae ?S. kudriavzevii hybrids experienced various modifications. However, the S. cerevisiae ? S.kudriavzevii F1 hybrids underwent more severe genomic alterations than the S. cerevisiae ?S. uvarum ones. Generation of the new genotypes also influenced the physiological performancesof the hybrids and the occurrence of novel phenotypes. Significant differencesin carbohydrate utilization and distinct growth dynamics at increasing concentrations ofsodium chloride, urea and miconazole were observed within and between the S. cerevisiae? S. uvarum and S. cerevisiae ? S. kudriavzevii hybrids. Parental strains also demonstrateddifferent contributions to the final metabolic outcomes of the hybrid yeasts. A comparisonof the genotypic properties of the artificial hybrids with several hybrid isolates from thewine-related environments and wastewater demonstrated a greater genetic variability ofthe S. cerevisiae ? S. kudriavzevii hybrids. Saccharomyces cerevisiae ? S. uvarum artificial andnatural hybrids showed considerable differences in osmolyte tolerance and sensitivity tomiconazole, whereas the S. cerevisiae ? S. kudriavzevii hybrids exhibited differences also inmaltotriose utilization. The results of this study suggest that chromosomal rearrangementsand genomic reorganizations as post-hybridization processes may affect the phenotypicproperties of the hybrid progeny substantially. Relative to their ancestors, the F1 segregants may generate different phenotypes, indicating novel routes of evolution in responseto environmental growth conditions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Food Technology and Biotechnology. - 52 : 1 (2014), p. 46-57. -
További szerzők:Atanasova, Lea Karanyicz Edina Sipiczki Mátyás (1948-) (biológus) Bond, Ursula Druzhinina, Irina S. Sterflinger, Katja Lopandic, Ksenija
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