Találati lista | Tudóstér

001-es BibID:	BIBFORM063758
Első szerző:	Olamendi-Portugal, Timoteo
Cím:	Isolation, chemical and functional characterization of several new K+-channel blocking peptides from the venom of the scorpion Centruroides tecomanus / Timoteo Olamendi-Portugal, Adam Bartok, Fernando Zamudio-Zuniga, Andras Balajthy, Baltazar Becerril, Gyorgy Panyi, Lourival D. Possani
Dátum:	2016
ISSN:	0041-0101
Megjegyzések:	AbstractSix new peptides were isolated from the venom of the Mexican scorpion Centruroides tecomanus; their primary structures were determined and the effects on ion channels were verified by patch-clamp experiments. Four are K(+)-channel blockers of the ?-KTx family, containing 32 to 39 amino acid residues, cross-linked by three disulfide bonds. They all block Kv1.2 in nanomolar concentrations and show various degree of selectivity over Kv1.1, Kv1.3, Shaker and KCa3.1 channels. One peptide has 42 amino acids cross-linked by four disulfides; it blocks ERG-channels and belongs to the ?-KTx family. The sixth peptide has only 32 amino acid residues, three disulfide bonds and has no effect on the ion-channels assayed. It also does not have antimicrobial activity. Systematic numbers were assigned (time of elution on HPLC): ?-KTx 10.4 (time 24.1); ?-KTx 2.15 (time 26.2); ?-KTx 2.16 (time 23.8); ?-KTx 2.17 (time 26.7) and ?-KTx 1.9 (elution time 29.6). A partial proteomic analysis of the short chain basic peptides of this venom, which elutes on carboxy-methyl-cellulose column fractionation, is included. The pharmacological properties of the peptides described in this study may provide valuable tools for understanding the structure-function relationship of K(+) channel blocking scorpion toxins.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Toxicon 115 (2016), p. 1-12. -
További szerzők:	Bartók Ádám (1984-) (biotechnológus) Zamudio, Fernando Z. Balajthy András (1988-) (általános orvos) Becerril, Baltazar Panyi György (1966-) (biofizikus) Possani, Lourival Domingos
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: