CCL

Összesen 5 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM049594
035-os BibID:PMID:23846866
Első szerző:Doan-Xuan, Quang-Minh (biofizikus)
Cím:High content analysis of differentiation and cell death in human adipocytes / Quang Minh Doan-Xuan, Anitta K. Sarvari, Pamela Fischer-Posovszky, Martin Wabitsch, Zoltan Balajthy, Laszlo Fesus, Zsolt Bacso
Dátum:2013
ISSN:1552-4922 1552-4930
Megjegyzések:Understanding adipocyte biology and its homeostasis is in the focus of current obesity research. We aimed to introduce a high-content analysis procedure for directly visualizing and quantifying adipogenesis and adipoapoptosis by laser scanning cytometry (LSC) in a large population of cell. Slide-based image cytometry and image processing algorithms were used and optimized for high-throughput analysis of differentiating cells and apoptotic processes in cell culture at high confluence. Both preadipocytes and adipocytes were simultaneously scrutinized for lipid accumulation, texture properties, nuclear condensation, and DNA fragmentation. Adipocyte commitment was found after incubation in adipogenic medium for 3 days identified by lipid droplet formation and increased light absorption, while terminal differentiation of adipocytes occurred throughout day 9-14 with characteristic nuclear shrinkage, eccentric nuclei localization, chromatin condensation, and massive lipid deposition. Preadipocytes were shown to be more prone to tumor necrosis factor alpha (TNFα)-induced apoptosis compared to mature adipocytes. Importantly, spontaneous DNA fragmentation was observed at early stage when adipocyte commitment occurs. This DNA damage was independent from either spontaneous or induced apoptosis and probably was part of the differentiation program.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
DNA damage
SGBS
adipocyte
differentiation
high content analysis
laser scanning cytometry
preadipocyte
Doktori iskola
Megjelenés:Cytometry Part A. - 83 : 10 (2013), p. 933-943. -
További szerzők:Sárvári Anitta Kinga (1984-) (biológus) Fischer-Posovszky Pamela Wabitsch, Martin Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus) Bacsó Zsolt (1963-) (biofizikus)
Pályázati támogatás:NK 105046
OTKA
OMFB-01626/2006
OTKA
GVOP-3.2.1-2004-04-0351/3.0
GVOP
TECH09-A2-2009-0131
GVOP
TRANSCOM IAPP 251506
FP7
NKTH NTP Schizo08
Egyéb
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
Molekuláris Sejt- és Immunbiológiai Doktori Iskola
TÁMOP-4.2.2.A-11/1/KONV-2012-0023-"VÉD-ELEM"
TÁMOP
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM065903
Első szerző:Kristóf Endre (általános orvos)
Cím:Clozapine modifies the differentiation program of human adipocytes inducing browning / Endre Kristóf, Quang-Minh Doan-Xuan, Anitta Kinga Sárvári, Ágnes Klusóczki, Pamela Fischer-Posovszky, Martin Wabitsch, Zsolt Bacso, Péter Bai, Zoltán Balajthy, László Fésüs
Dátum:2016
Megjegyzések:Administration of second-generation antipsychotic drugs (SGAs) often leads to weight gain and consequent cardio-metabolic side effects. We observed that clozapine but not 6 other antipsychotic drugs reprogrammed the gene expression pattern of differentiating human adipocytes ex vivo, leading to an elevated expression of the browning marker gene UCP1, more and smaller lipid droplets and more mitochondrial DNA than in the untreated white adipocytes. Laser-scanning cytometry showed that up to 40% of the differentiating single primary and SGBS adipocytes had the characteristic morphological features of browning cells. Furthermore, clozapine significantly up-regulated ELOVL3, CIDEA, CYC1, PGC1A and TBX1 genes but not ZIC1 suggesting induction of the beige-like and not the classical brown phenotype. When we tested if browning induced by clozapine can be explained by its known pharmacological effect of antagonizing serotonin (5HT) receptors it was found that browning cells expressed 5HT receptors 2A, 1D, 7 and the up-regulation of browning markers was diminished in the presence of exogenous 5HT. Undifferentiated progenitors or completely differentiated beige or white adipocytes did not respond to clozapine administration. The clozapine induced beige cells displayed increased basal and oligomycin inhibited (proton leak) oxygen consumption but these cells showed a lower response to cAMP stimulus as compared to control beige adipocytes indicating that they are less capable to respond to natural thermogenic anti-obesity cues. Our data altogether suggest that novel pharmacological stimulation of these masked beige adipocytes can be a future therapeutic target for treatment of SGA-induced weight gain.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
adipocita
beige
clozapine
lézer-pásztázó citometria
elhízás
szerotonin
termogenezis
Megjelenés:Translational Psychiatry. - 6 : 11 (2016), p. 1-12. -
További szerzők:Doan-Xuan, Quang-Minh (1986-) (biofizikus) Sárvári Anitta Kinga (1984-) (biológus) Klusóczki Ágnes (1991-) (biotechnológus) Fischer-Posovszky Pamela Wabitsch, Martin Bacsó Zsolt (1963-) (biofizikus) Bai Péter (1976-) (biokémikus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:TÁMOP-4.2.4.A/2-11/1-2012-0001
TÁMOP
TÁMOP-4.2.2.A-11/1/KONV-2012-0023
TÁMOP
TÁMOP-4.2.2.A-11/1/KONV-2012-0025
TÁMOP
TRANSCOM IAPP 251506
FP7
MTA-DE
MTA
Apoptózis és Genomika Kutatócsoport
K108308
OTKA
NK105046
OTKA
GINOP-2.3.2-15-2016-00006
GINOP
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM060597
Első szerző:Kristóf Endre (általános orvos)
Cím:Second generation antipsychotic (SGA) drugs modify the differentiation program of human adipocytes inducing 'browning' markers / E. Kristof, D. Minh, A. Sarvari, Z. Balajthy, Z. Bacso, L. Fesus
Dátum:2013
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:FEBS journal 280 : 1 (2013), p. 289. -
További szerzők:Doan-Xuan, Quang-Minh (1986-) (biofizikus) Sárvári Anitta Kinga (1984-) (biológus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Bacsó Zsolt (1963-) (biofizikus) Fésüs László (1947-) (orvos biokémikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:

4.

001-es BibID:BIBFORM056693
Első szerző:Sárvári Anitta Kinga (biológus)
Cím:Interaction of differentiated human adipocytes with macrophages leads to trogocytosis and selective IL-6 secretion / A. K. Sárvári, Quang-Minh Doan-Xuan, Z. Bacsó, I. Csomós, Z. Balajthy, L. Fésüs
Dátum:2015
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Cell Death and Disease. - 6 (2015), p. e1613. -
További szerzők:Doan-Xuan, Quang-Minh (1986-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Csomós István (1983-) (molekuláris biológus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM056375
Első szerző:Sárvári Anitta Kinga (biológus)
Cím:Atypical antipsychotics induce both proinflammatory and adipogenic gene expression in human adipocytes in vitro / Anitta K. Sárvári, Zoltán Veréb, Iván P. Uray, László Fésüs, Zoltán Balajthy
Dátum:2014
ISSN:0006-291X
Megjegyzések:Schizophrenia requires lifelong treatment, potentially causing systemic changes in metabolic homeostasis. In the clinical setting, antipsychotic treatment may differentially lead to weight gain among individual patients, although the molecular determinants of such adverse effects are currently unknown. In this study, we investigated changes in the expression levels of critical regulatory genes of adipogenesis, lipid metabolism and proinflammatory genes during the differentiation of primary human adipose-derived stem cells (ADSCs). These cells were isolated from patients with body mass indices <25 and treated with the second-generation antipsychotics olanzapine, ziprasidone, clozapine, quetiapine, aripiprazole and risperidone and the first-generation antipsychotic haloperidol. We found that antipsychotics exhibited a marked effect on key genes involved in the regulation of cell cycle, signal transduction, transcription factors, nuclear receptors, differentiation markers and metabolic enzymes. In particular, we observed an induction of the transcription factor NF-KB1 and NF-KB1 target genes in adipocytes in response to these drugs, including the proinflammatory cytokines TNF-α, IL-1β, IL-8 and MCP-1. In addition, enhanced secretion of both IL8 and MCP-1 was observed in the supernatant of these cell cultures. In addition to their remarkable stimulatory effects on proinflammatory gene transcription, three of the most frequently prescribed antipsychotic drugs, clozapine, quetiapine and aripiprazole, also induced the expression of essential adipocyte differentiation genes and the adipocyte hormones leptin and adiponectin, suggesting that both glucose and fat metabolism may be affected by these drugs. These data further suggest that antipsychotic treatments in patients alter the gene expression patterns in adipocytes in a coordinated fashion and priming them for a low-level inflammatory state.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical and Biophysical Research Communications. - 450 : 4 (2014), p. 1383-1389. -
További szerzők:Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Uray Iván Péter (1970-) (kutatóorvos) Fésüs László (1947-) (orvos biokémikus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1