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001-es BibID:	BIBFORM099343
035-os BibID:	(WoS)000608018200036 (Scopus)85097787135
Első szerző:	Hámori Csaba (vegyész)
Cím:	Colorado potato beetle alpha-amylase: Purification, action pattern and subsite mapping for exploration of active centre / Hámori Csaba, Remenyik Judit, Kandra Lili, Gyémánt Gyöngyi
Dátum:	2021
ISSN:	0141-8130
Megjegyzések:	Colorado potato beetle is an invasive insect herbivore and one of the most challenging agricultural pests globally. This study is the first characterization of the active centre of Colorado potato beetle (Leptinotarsa decemlineata) alpha-amylase (LdAmy). Bond cleavage frequency values for LdAmy were determined by HPLC product analysis on a chromophore labelled maltooligomer substrate series. Binding energies between amino acid moieties of subsites and glucose residues of substrate were calculated. Active site contains six subsites in the binding region of LdAmy; four glycone- (-4, -3, -2, -1) and two aglycone-binding sites (+1, +2). Subsite map calculation resulted in apparent binding energies -11.8 and - 11.0 kJ/mol for subsites (+2) and (-3), respectively, which revealed very favorable interactions at these positions. Structures of binding sites of LdAmy and mammalian a-amylases show similarity, but there are variations in the binding energies at subsite (-2) and (-4). Differences were interpreted by comparison of amino acid sequences of human salivary alpha-amylase (HSA) and porcine pancreatic alpha-amylase (PPA) and two insect (Leptinotarsa decemlineata and Tenebrio molitor) enzymes. The observed substitution of positively charged His305 in HSA at subsite (-2) with an acidic Asp in LdAmy in the same position may explain the obtained energy reduction.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	International Journal Of Biological Macromolecules. - 168 (2021), p. 350-355. -
További szerzők:	Gálné Remenyik Judit (1965-) (kémia tanár, okleveles vegyész) Kandra Lili (1943-) (biokémikus) Gyémánt Gyöngyi (1960-) (vegyész)
Pályázati támogatás:	GINOP-2.3.2- 15-2016-00008 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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