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001-es BibID:	BIBFORM106511
035-os BibID:	(WoS)000995038700001 (Scopus)85147089622
Első szerző:	Freytag Csongor (biológus)
Cím:	B" and C subunits of PP2A regulate the levels of reactive oxygen species and superoxide dismutase activities in Arabidopsis / Csongor Freytag, Tamas Garda, Zoltan Konya, Marta M-Hamvas, Balazs Toth-Varady, Gabriella Petra Juhasz, Laszlo Ujlaky-Nagy, Adrienn Kelemen, Gabor Vasas, Csaba Mathe
Dátum:	2023
ISSN:	0981-9428
Megjegyzések:	The serine-threonine protein phosphatases PP2A regulate many cellular processes, however their role in oxidative stress responses and defence is less known. We show the involvement of its C (catalytic) and B" (a regulatory) subunits. The c3c4 (C subunit) and fass (B") subunit mutants and Col wt of Arabidopsis were used. Controls and treatments with the PP2A inhibitor microcystin-LR (MCY-LR) and reactive oxygen species (ROS) inducer diquat (DQ) were employed. ROS levels of primary roots were largely genotype dependent and both C and B" subunit mutants had increased sensitivity to MCY-LR and DQ indicating the involvement of these subunits in oxidative stress induction. Superoxide dismutases (SOD), mainly the Cu/Zn-SOD isoform, as key enzymes involved in ROS scavenging are also showing altered (mostly increased) activities in both c3c4 and fass mutants and have opposite relations to ROS induction. This indicates that the two types of subunits involved have partially different regulatory roles. In relation to this, control and MCY-LR/DQ treated B" subunit mutants were proven to have altered levels of phosphorylation of histone H2AX. γH2AX, the phosphorylated form indicates double stranded DNA damage during oxidative stress. Overall we point out the probable pivotal role of several PP2A subunits in the regulation of oxidative stress responses in plants and pave the way for future research to reveal the signaling pathways involved.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Protein phosphatase PP2A B" subunit C3C4 subunits Oxidative stress Phosphorylated H2AX Superoxide dismutase Cu/Zn-SOD
Megjelenés:	Plant Physiology And Biochemistry. - 195 (2023), p. 182-192. -
További szerzők:	Garda Tamás (1990-) (biológus) Kónya Zoltán (1986-) (molekuláris biológus, biokémikus) Mikóné Hamvas Márta (1963-) (biológus) Tóth-Várady Balázs Juhász Gabriella Petra (1994-) (biologia tanár) Ujlaky-Nagy László (1977-) (biofizikus) Kelemen Adrienn (1995-) (Biológus) Vasas Gábor (1975-) (biológus-vegyész) Máthé Csaba (1966-) (biológus)
Pályázati támogatás:	120638 OTKA 119647 OTKA ÚNKP-22-4-I-DE-11 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM109570
035-os BibID:	(cikkazonosító)586 (Scopus)85151426418 (WoS)000956870300001
Első szerző:	Juhász Gabriella Petra (biologia tanár)
Cím:	Microcystin-LR, a Cyanobacterial Toxin, Induces Changes in the Organization of Membrane Compartments in Arabidopsis / Gabriella Petra Juhász, Sándor Kéki, Anita Dékány-Adamoczky, Csongor Freytag, Gábor Vasas, Csaba Máthé, Tamás Garda
Dátum:	2023
ISSN:	2076-2607
Megjegyzések:	To evaluate the effects of the cyanobacterial toxin microcystin-LR (MCY-LR, a protein phosphatase inhibitor) and diquat (DQ, an oxidative stress inducer) on the organization of tonoplast, the effect of MCY-LR on plastid stromule formation and on mitochondria was investigated in wildtype Arabidopsis. Tonoplast was also studied in PP2A catalytic (c3c4) and regulatory subunit mutants (fass-5 and fass-15). These novel studies were performed by CLSM microscopy. MCY-LR is produced during cyanobacterial blooms. The organization of tonoplast of PP2A mutants of Arabidopsis is much more sensitive to MCY-LR and DQ treatments than that of wild type. In c3c4, fass-5 and fass-15, control and treated plants showed increased vacuole fragmentation that was the strongest when the fass-5 mutant was treated with MCY-LR. It is assumed that both PP2A/C and B" subunits play an important role in normal formation and function of the tonoplast. In wild-type plants, MCY-LR affects mitochondria. Under the influence of MCY-LR, small, round-shaped mitochondria appeared, while long/fused mitochondria were typical in control plants. Presumably, MCY-LR either inhibits the fusion of mitochondria or induces fission. Consequently, PP2A also plays an important role in the fusion of mitochondria. MCY-LR also increased the frequency of stromules appearing on chloroplasts after 1 h treatments. Along the stromules, signals can be transported between plastids and endoplasmic reticulum. It is probable that they promote a faster response to stress.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Microorganisms. - 11 : 3 (2023), p. 1-13. -
További szerzők:	Kéki Sándor (1964-) (polimer kémikus) Dékány-Adamoczky Anita (1985-) (anyagmérnök) Freytag Csongor (1993-) (biológus) Vasas Gábor (1975-) (biológus-vegyész) Máthé Csaba (1966-) (biológus) Garda Tamás (1990-) (biológus)
Pályázati támogatás:	OTKA-120638 OTKA OTKA-132685 OTKA TKP2021-EGA-20 Egyéb ÚNKP-22-4-I-DE-11 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM110538
Első szerző:	Máthé Csaba (biológus)
Cím:	New insights into the roles of PP2A subunits FASS and C3/C4 in the regulation of mitosis and oxidative stress responses in Arabidopsis / Csaba Máthé, Zoltán Kónya, Adrienn Kelemen, Tamás Garda, Márta M-Hamvas, Gabriella Petra Juhász, Csongor Freytag
Dátum:	2023
Megjegyzések:	PP2A, a serine-threonine protein phosphatase is a complex consisting of A (scaffolding), B (regulatory) and C (catalytic) subunits. By using wild-type and loss-of-function phosphatase-related mutants (fass for a B" and c3c4 for two catalytic subunit isoforms that interact with Fass in wild-type plants) of Arabidopsis, we have found that as expected, C3/C4 subunits are responsible for a significant proportion of total PP2A activity, and surprisingly affect the activity of a related phosphatase, PP1 as well. We show for the first time that fass mutants are not impaired significantly in total phosphatase activity, because instead of decreasing PP2A, the balance between PP2A-PP1 activities is altered. Immunohistochemical studies for microtubules and phospho-histone H3 show that both types of PP2A subunits regulate mitotic activity in the root apical meristem and influence the phosphorylation of histone H3 during mitosis, a process important in the regulation of metaphase-anaphase transition and the entire mitotic process. Fass exerts this function not only by regulating PP2A (possibly involving its subcellular localization), but also by influencing PP1 activity. The two types of subunits are affecting reactive oxygen species (ROS) production (histochemical studies) and scavenging as well. By studying the activities of different superoxide dismutase (SOD) isoforms, we found that the primary target of Fass and in relation to this, C3 and C4 is Cu/Zn SOD. The protein phosphatase inhibitor microcystin-LR increased the susceptibility of mutants to both mitotic anomalies and alterations of ROS metabolism.
Tárgyszavak:	Biológiai tudományok Természettudományok előadáskivonat könyvrészlet
Megjelenés:	Botanical Microscopy Meeting 2023 / Kim Findlay, Christine Faulkner; Patrick Hussey; Ulla Neumann; Richard Smith; Imogen Sparkes. - p. 77-78.
További szerzők:	Kónya Zoltán (1986-) (molekuláris biológus, biokémikus) Kelemen Adrienn (1995-) (Biológus) Garda Tamás (1990-) (biológus) Mikóné Hamvas Márta (1963-) (biológus) Juhász Gabriella Petra (1994-) (biologia tanár) Freytag Csongor (1993-) (biológus)
Pályázati támogatás:	K120638 OTKA
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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