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001-es BibID:	BIBFORM064493
035-os BibID:	(cikkazonosító)1047 (scopus)85016422224 (wos)000381500900062
Első szerző:	Szemán-Nagy Gábor (biológia tanár-molekuláris biológus)
Cím:	In situ electron microscopy of lactomicroselenium particles in probiotic bacteria / Nagy Gábor, Pinczes Gyula, Pintér Gábor, Pócsi István, Prokisch József, Bánfalvi Gáspár
Dátum:	2016
ISSN:	1661-6596 1422-0067
Megjegyzések:	Electron microscopy was used to test whether or not (a) in statu nascendi synthesized, and in situ measured, nanoparticle size does not differ significantly from the size of nanoparticles after their purification; and (b) the generation of selenium is detrimental to the bacterial strains that produce them. Elemental nano-sized selenium produced by probiotic latic acid bacteria was used as a lactomicroselenium (lactomicroSel) inhibitor of cell growth in the presence of lactomicroSel, and was followed by time-lapse microscopy. The size of lactomicroSel produced by probiotic bacteria was measured in situ and after isolation and purification. For these measurements the TESLA BS 540 transmission electron microscope was converted from analog (aTEM) to digital processing (dTEM), and further to remote-access internet electron microscopy (iTEM). Lactobacillus acidophilus produced fewer, but larger, lactomicroSel nanoparticles (200?350 nm) than Lactobacillus casei (L. casei), which generated many, smaller lactomicroSel particles (85?200 nm) and grains as a cloudy, less electrodense material. Streptococcus thermophilus cells generated selenoparticles (60-280 nm) in a suicidic manner. The size determined in situ in lactic acid bacteria was significantly lower than those measured by scanning electron microscopy after the isolation of lactomicroSel particles obtained from lactobacilli (100?500 nm), but higher relative to those isolated from Streptococcus thermopilus (50?100 nm). These differences indicate that smaller lactomicroSel particles could be more toxic to the producing bacteria themselves and discrepancies in size could have implications with respect to the applications of selenium nanoparticles as prebiotics.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban transmission electronmicroscopy lactomicroSel nanoparticles lactobacilli Streptococcus thermophilus S. thermophilus
Megjelenés:	International Journal of Molecular Sciences. - 17 : 7 (2016), p. 1-8. -
További szerzők:	Pinczes Gyula Pintér Gábor Pócsi István (1961-) (vegyész) Prokisch József (1966-) (vegyész) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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