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001-es BibID:	BIBFORM066378
Első szerző:	Kristóf Endre (általános orvos)
Cím:	Differentiating human beige adipocytes secrete cytokines ("batokines") / Endre Kristóf, Ágnes Klusóczki, Zsolt Combi, Roland Veress, Ferenc Győry, Szilárd Póliska, Zsolt Bacsó, László Fésüs
Dátum:	2016
Megjegyzések:	Several studies highlighted the strong negative correlation between obesity and metabolically active brown adipose tissue amount in adult humans. There are at least two types of thermogenic fat depots, classical brown and beige, which have different origins and tissue distribution. Beige adipocytes contribute to the regulation of systemic metabolic homeostasis not exclusively by thermogenesis and mitochondrial uncoupling. In the present study, we investigated the secretion of "batokines" by primary human brown and beige adipocytes and by human adipose tissue samples. Adipose-derived mesenchymal stem cells obtained from herniotomy (abdominal subcutaneous) or thyroid surgery ("deep neck" and cervical subcutaneous) and a human preadipocyte cell line (SGBS) were differentiated into white, brown (by BMP7 treatment) or beige (by irisin administration or by a previously described cocktail) adipocytes. Cytokine gene expression in browning adipocytes was determined by RNA sequencing. Conditioned differentiation media were collected during the replacement of the adipogenic cocktails and secreted IL-6, IL-8, TNF?, MCP-1 and IL1-? were measured by ELISA. IL-6, MCP-1 and IL-8 secretion was significantly higher by beige compared to white adipocytes. In contrast to BMP7 administration (when classical brown adipocyte differentiation occurs), irisin treatment (which induces beige adipocyte differentiation) resulted in an increased total IL-6, MCP-1 and IL-8 production. Media collected daily or after three day periods contained the same amount of IL-6 depending only on the phase of differentiation. This suggests that adipocytes adjusted their production of the cytokine to reach an optimal level in the medium. In the heterogeneous population of differentiating adipocytes, IL-6 containing vesicles could be visualized mostly in beige cells by Laser-scanning cytometry. In the future we intend to explore the functional significance of the produced "batokines".
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:	Biokémia. - 40 : 3 (2016), p. 37. -
További szerzők:	Klusóczki Ágnes (1991-) (biotechnológus) Combi Zsolt (1981-) Veress Roland (1992-) (molekuláris biológus) Győry Ferenc (1969-) (kardiológus) Póliska Szilárd (1978-) (biológus) Bacsó Zsolt (1963-) (biofizikus) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	TÁMOP-4.2.2.A-11/1/KONV-2012-0023 TÁMOP TÁMOP-4.2.2.A-11/1/KONV-2012-0025 TÁMOP TRANSCOM IAPP 251506 FP7 MTA-DE MTA Apoptózis és Genomika Kutatócsoport NK105046 OTKA GINOP-2.3.2-15-2016-00006 GINOP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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