CCL

Összesen 7 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM071245
Első szerző:Madar Anett
Cím:Conserved transcriptional regulators in the Schizosaccharomyces group / Anett Madar, Emese Pataki, Ida Miklós, I. J. Holb, M. Sipiczki
Dátum:2012
Megjegyzések:The Schizosaccharomyces group consists of four species: Sch. pombe, Sch. japonicus, Sch. octosporus, and Sch.cryophilus.Their cells are eukaryotic and haploid, which divide by medial fission. Their genomes have been sequenced(http://www.broadinstitute.org) and the data enable us to compare their genes, proteins and cellular processes. As the fissionyeast cells grow fast, it makes these microorganisms very suitable for breeding. These features give attractive possibilities ofcombining the bioinformatic methods with experimental research. Our research group is interested in studying of celldivision, with special emphasis on cytokinesis and its transcriptional regulation. Therefore, our aim was to gain informationabout the sequence- and functional homology of transcriptional regulators of the cell separation. After bioinformatic analysisof the proteins, we have found two proteins, which showed high degree of homology. Rsv1: Identities=99/167 (59%),Positives=120/167 (71%). Med31: Score = 214.927 (546), Expect=0.0 Identities=98/108 (90%), Positives=104/108 (96%). Inthe med31 protein we have found 37 of 139a.acids, which are constant from yeasts to human. The interspecificcomplementation of these genes has been carried out. Namely, the cloned genes of Sch. octosporus have been transformedinto the mutant cells of Sch. pombe. The investigation of phenotypic features of the transformant cells has revealed functionalhomology between the genes.
Tárgyszavak:Természettudományok Biológiai tudományok előadáskivonat
Megjelenés:A Magyar Mikrobiológiai Társaság 2012. évi Nagygyűlése : Absztraktfüzet. - p. 29.
További szerzők:Pataki Emese (1989-) (molekuláris biológus) Miklós Ida (1962-) (biológus) Holb Imre (1973-) (agrármérnök) Sipiczki Mátyás (1948-) (biológus)
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM071241
Első szerző:Pataki Emese (molekuláris biológus)
Cím:Interspecific complementation analysis between Schizosaccharomyces pombe and Candida albicans / Emese Pataki, Mátyás Sipiczki, Ida Miklós
Dátum:2015
ISSN:1217-8950 1588-2640
Megjegyzések:Candida albicans is the most common dimorphic human fungal pathogen. The pathogenesis of itsinfection is poorly understood. A recent study showed that Cas5 played an important role in thepathogenesis of Candida albicans infections and it revealed that Cas5 is required for expression ofcaspofungin-inducible genes, many of which had connection to cell wall integrity. Since defense ofthe host immune system based on the recognition of conserved molecular patterns of the fungal cellwall, deficiencies in the cell wall structure might promote the recognition and elimination ofCandida albicans by host immune cells. Cas5+ encodes a zink-finger transcription factor that ispartially homologous with rsv1+ gene of Schizosaccharomyces group. Based on the sequencesimilarity it is presumable, that rsv1 and Cas5 have the same function. To prove their functionalhomology, we started the interspecific complementation analysis. Namely, we amplified the Cas5+gene by PCR and cloned into S. pombe nmt promoter containing overexpression vector. This DNAconstruction was transformed into the S. pombe rsv1 mutant strain. The characterization and thecaspofungin sensitivity of the transformants are in progress.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 62 : Suppl. (2015), p. 197. -
További szerzők:Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM071242
Első szerző:Pataki Emese (molekuláris biológus)
Cím:fhl1 controls transcription of the nitrogen starvation induced genes in cooperation with TORC1 in Schizosaccharomyces pombe / Emese Pataki, Ronit Weisman, Mátyás Sipiczki, Ida Miklós
Dátum:2015
ISSN:1217-8950 1588-2640
Megjegyzések:One of the most important regulators of cellular processes is the protein kinase TOR (Target ofRapamycin), which is conserved from yeast to humans. TOR is a protein kinase that was isolated asthe target of the immunosuppressive and anticancer drug rapamycin. It regulates the response toenvironmental changes and cellular energy status, cell growth, cell proliferation, cell survival,protein synthesis, and transcription. In the fission yeast, Schizosaccharomyces pombe there are twoTOR homologs. Tor1 is part of a protein complex known as TORC2, while Tor2 is part of a proteincomplex known as TORC1. These complexes were found to regulate oppositely the response tonutrient depletion, in particular the response to nitrogen starvation. TORC1 is essential and playsmajor role in the control of cellular growth, while TORC2 regulates cell survival under stressconditions. Here we demonstrate that overexpression of fhl1+ gene suppresses the mRNA level ofthe nitrogen starvation response genes: mei2, ste4, ste11 and decreases the mating efficiency of tor2-ts mutant. Moreover overexpression of fhl1+ modifies the stress response of tor2-ts mutant, amongothers in the presence of rapamycin.Based on these findings we can conclude that fhl1 plays a role in the TOR pathway and tor2controls the response to nitrogen starvation via fhl1 transcription factor.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 62 : Suppl. (2015), p. 197-197. -
További szerzők:Weisman, Ronit Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

4.

001-es BibID:BIBFORM071243
Első szerző:Pataki Emese (molekuláris biológus)
Cím:Microarray analysis of a forkhead transcription factor of Schizosaccharomyces pombe / Emese Pataki, Ida Miklos, Matthias Sipiczki
Dátum:2013
ISSN:1217-8950 1588-2640
Megjegyzések:The forkhead proteins are a family of the transcription factors that play important roles in regulatingthe expression of genes, involved in cell growth, cell division, sexual differentiation or in embryonicdevelopment. [1] [2] The forkhead family of proteins contain a highly conserved Fork Head domainthat are widespread in eukaryotes, found throughout yeast and human.To understand the process of regulating of forkhead transcription factors, researchers use modelorganisms, such as Saccharomyces cerevisiae or the fission yeast Schizosaccharomyces pombe.Earlier we started to characterize the Fhl1 forkhead gene of Sch. pombe. It revealed that deletion ofthis gene lead to slow cell growth, delayed G2-to-M transition and modify the response ofenvironmental stresses[1]. Our aim was to carry on these experiments and gain more informationabout function of the Sch. pombe Fhl1 gene. A microarray analysis of the Fhl1 deleted strain wascarried out. Our results suggest that Fhl1 gene display a remarkable function in meiosis as regulatesthe ste11 gene. It also influences the transport mechanisms and kinases. Its target genes can befound at all chromosomes. Both down- and up regulated genes appeared among its targets.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 60 : Suppl. (2013), p. 212-213. -
További szerzők:Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM071244
Első szerző:Pataki Emese (molekuláris biológus)
Cím:rsv1 gene has functional homology in the Schizosaccharomyces species / Emese Pataki, Ida Miklos, Matthias Sipiczki
Dátum:2013
ISSN:1217-8950 1588-2640
Megjegyzések:Transcriptional regulators are involved in the regulation of gene expression. They are usually boundto the special sites of DNA sequence and hereby allow for a sophisticated response to multipleconditions in the environment. One of the transcriptional regulatory proteins is the rsv1, which ispartly characterised in Sch. pombe. It is a negative regulator of cellular carbohydrate metabolicprocess. It encodes a zinc finger protein, which is required for cell viability in stationary phaseinduced by glucose exhaustion. Cells lacking rsv1+ are viable and the gene is regulated by pka1. [1]The four fission yeast species form an early-branching clade among the Ascomycete. One of them isthe Sch. pombe, which is a well-known model organism. Its relatives, such as Sch. japonicus, Sch.octosporus or Sch. cryophilus can also be attractive models of the genetic studies. We wanted to getinformation about the role of Sch. octosporus rsv1 gene. As there is not rsv1 mutant strain at thismoment in this species, its characterisation is impossible. Therefore, we used the quantification ofsequence similarity and interspecific complementation analysis to prove the function of Sch.octosporus rsv1 gene. The BLASTp analysis suggested, that SOCG_03341 gene must be the rsv1gene. However, sequence-based functional predictions are reliable, not all homologs have the samefunction. Therefore, we cloned the Sch. octosporus rsv1 gene into a Sch. pombe vector, and thisDNA construction was transformed into the Sch. pombe rsv1 mutant strain.The characterisation of the transformants proved the functional homology between the Sch. pombersv1 and Sch. octosporus SOCG_03341 gene.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 60 : Suppl. (2013), p. 213. -
További szerzők:Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

6.

001-es BibID:BIBFORM070494
Első szerző:Pataki Emese (molekuláris biológus)
Cím:Schizosaccharomyces pombe rsv1 Transcription Factor and its Putative Homologues Preserved their Functional Homology and are Evolutionarily Conserved / Emese Pataki, Matthias Sipiczki, Ida Miklos
Dátum:2017
ISSN:0343-8651 1432-0991
Megjegyzések:Environmental glucose is an important regulator of biological processes, as it can launch different cell processes depending on its concentration. Thus, low glucose concentration can induce entry into quiescence, which ensures long-term viability for the cells or in other cases mycelial growth in the dimorphic species, which, in turn, provides the cells with fresh nutrients. Several genes, such as the genes of cAMP cascade, are involved in glucose sensing and response. Since this signal transduction pathway seemed to be an evolutionarily conserved process, we assumed that its genes were also conserved and preserved their functional homology. To obtain evidence, Schizosaccharomyces pombe rsv1 and its orthologous genes were investigated using in silico and experimental approaches. Our results supported that the Rsv1 zinc-finger transcription factors of Schizosaccharomyces japonicus and Schizosaccharomyces octosporus and the Candida albicans cas5p were really functional homologues of the S. pombe Rsv1. Namely, the homologous proteins were able to restore mutant phenotype of the S. pombe rsv1-deleted cells. Bioinformatic anaysis revealed that the most conserved parts of the proteins always contained the C2H2 domains and the complementation abilities of the counterpart genes were not uniform regarding the investigated features, which can be in connection with the conserved regions outside C2H2.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Schizosaccharomyces pombe
zink finger transcription factor
functional homology
Megjelenés:Current Microbiology 74 : 6 (2017), p. 710-717. -
További szerzők:Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA-106172
OTKA
European Social Fund
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

7.

001-es BibID:BIBFORM070493
Első szerző:Pataki Emese (molekuláris biológus)
Cím:fhl1 gene of the fission yeast regulates transcription of meiotic genes and nitrogen starvation response, downstream of the TORC1 pathway / Emese Pataki, Ronit Weisman, Matthias Sipiczki, Ida Miklos
Dátum:2016
ISSN:0172-8083 1432-0983
Megjegyzések:Environmental changes, such as nutrient limitation or starvation induce different signal transducing pathways, which require coordinated cooperation of several genes. Our previous data revealed that the fhl1 fork-head type transcription factor of the fission yeast could be involved in sporulation, which was typically induced under poor conditions. Since the exact role of Fhl1 in this process was not known, we wanted to identify its downstream targets and to investigate its possible cooperation with another known regulator of sporulation. Gene expression and Northern blot analysis of the fhl1? mutant strain revealed the target genes involved in mating and sporulation. Our results also showed that Fhl1 could regulate nutrient sensing, the transporter and permease genes. Since the majority of these genes belonged to the nitrogen starvation response, the possible cooperation of fhl1 and tor2 was also investigated. Comparison of their microarray data and the expression of fhl1 + from a strong promoter in the tor2-ts mutant cells suggested that one part of the target genes are commonly regulated by Fhl1 and Tor2. Since the expression of fhl1 + from a strong promoter could rescue rapamycin and temperature sensitivity and suppressed the hyper-sporulation defect of the tor2-ts mutant cells, we believe that Fhl1 acts in TOR signaling, downstream of Tor2. Thus, this work shed light on certain novel details of the regulation of the sexual processes and a new member of the TOR pathway, but further experiments are needed to confirm the involvement of Fhl1 in nutrient sensing.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Schizosaccharomyces pombe
TOR pathway
Fork-head transcription factor
nitrogen response
Megjelenés:Current Genetics 63 : 1 (2016), p. 91-101. -
További szerzők:Weisman, Ronit Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA-106172
OTKA
TÁMOP 4.2.2.B-15/1/KONV-2015-0001.
TÁMOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1